Photosynthesis Lab

Name
Biology-Honors
Teacher
December 6, 2012
Photosynthesis Lab 1. Introduction: Light dependent reactions are the first phase of photosynthesis. It requires light to happen and happens in the thylakoid membrane in a chloroplast. The light energy is absorbed from the sun and converted into chemical energy. Which is then stored temporarily in ATP and NADPH.
Hypothesis: I predict that the wavelength intensity will affect the rate of photosynthesis more than light intensity will.

2. Data: # ATP | Light Intensity (Lux) | Time Elapsed | 1 | 40 | 0:04 | 1 | 80 | 0:04 | 2 | 80 | 2:10 | 1 | 120 | 0:04 | 2 | 120 | 1:27 | 3 | 120 | 2:51 | 1 | 160 | 0:04 | 2 | 160 | 1:06 | 3 | 160 | 2:09 | # ATP (produced at 3:00 minutes) | Light Intensity | 1 | 40 | 2 | 80 | 3 | 120 | 3 | 160 |

Data Continued:

# ATP | wavelength change (nm) | Time (hr: s) | 1 | 400 | 0:04 | 2 | 400 | 0:22 | 3 | 400 | 0:41 | 4 | 400 | 0:59 | 5 | 400 | 1:18 | 6 | 400 | 1:37 | 7 | 400 | 1:56 | 8 | 400 | 2:14 | 9 | 400 | 2:32 | 10 | 400 | 2:51 | 1 | 500 | 0:04 | 2 | 500 | 2:44 | 1 | 600 | 0:04 | 2 | 600 | 1:05 | 3 | 600 | 2:04 | 4 | 600 | 3:04 | 1 | 700 | 0:04 | 2 | 700 | 4:05 |

#ATP | Wavelength | 10 | 400 | 2 | 500 | 4 | 600 | 2 | 700 |

3. Graphs:

Graphs Continued:

4. Conclusion: Altering wavelength created a change in ATP synthesis, where 400 nm light allowed for the most ATP. Altering light intensity had no obvious effect and ATP production hovered at 1-3. My prediction was correct, with wavelength having a greater effect on ATP production than light