Purpose or Objectives: Observe several changes in matter and write questions concerning the properties of the samples.…
The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4, 0.8, 1.2, 1.6, 2.0 mg/ml of bovine serum were used to determine absorbance with a spectrophotometer. Two additional samples were made; one was blank and the other was for the specific homogenate sample. Then 3 microliters of bradford assay reagent, which indicates the amount of protein present by color, was added to all samples. The spectrophotometer was zeroed at 595 nm. A standard curve was made with the different absorbencies and concentrations. After the linear equation was formed, the unknown sample concentration was determined using the standard curve equation. A Gel Electrophoresis was used to perform a qualitative analysis. The use of 5 microliters of the homogenate was heated to 80 degrees Celsius. Then the homogenate was transferred to a 2-microliter-protein gel sample buffer. Samples loaded on to the gel was run at 100 v and stained with comassie blue; observations were made next lab. (Clendening 2014)…
After completing this experiment several of the chemicals just simply changed colors. The main colors shown were blue, yellow, pink, green, and a brownish black. A few of the reactants did not change to a different color at all, but did show little signs of bubbles at the surface.…
The experiment performed in the second part of the lab was heating 4 different chemical substances (s) and observing the changes made to them. Also determining if a chemical change had occurred. One test was performed with the substances in the tubes and another with the chemicals outside of the tubes and over the open flame.…
Purpose: To observe some properties of chemical reactions and to associate chemical properties with household products.…
Observations from procedure: BTB remains blue when exposed to a base and turns yellow when exposed to an acid. Phenolphthalein turns grey when exposed to a base and pink when exposed to an acid. Both chemicals turn a murky or foggy green when neutral.…
|Light Condition |Final pH |Water Movement (GROUP |Water Movement (CLASS |Photosynthesis Rate |Photosynthesis Rate |…
Please note that this procedure was adapted from the “Observation of Chemical and Physical Changes” LabPaq Lab manual (Hands-On Labs Version 42-01-82-00-03, 2015)…
The number of electrons forming a charge cloud around the nucleus is (pick one of the following) greater than; equal to; smaller than the number of protons in the nucleus of the atom.…
4. I will use goggles and cloves all the time, I also need to point dangerous materials away from other people and I should be specially cautious with hydrochloric acid.…
First, I would take a sample from different areas of the water to test the amount of dissolved oxygen in the water. Then after completing this test, I would check to see if there is” of course “an increase in the fish present in the water. This observation would help keep track of the fish present in different areas of the water and furthermore, I would be able to compare results.…
For the group of students who rested first, the average clothespin squeezes per minute (cps/min) were 142, 172, and 190.42. Combined the overall average for this group was 168.14 cps/min.…
One way to test a material for the presence of sodium bicarbonate is to take a sample and add HCl (Hydrochloric Acid) and see if a chemical reaction (bubbling, releasing Carbon Dioxide) occurs. If this chemical reaction does occur, then the material does in fact contain Sodium Bicarbonate.…
Yes. My hypothesis was the chocolate bar will gradually decrease in density as it gets smaller. According to the chart below, not only did the density decrease, but the mass and volume decreased as well. To reiterate, my hypothesis agreed with the results of my experiment.…
Abstract: The purpose of this experiment is to study the energy changes associated with the phase changes of water. In this experiment we know the water is gaining energy so we know the heat is going to be positive and we can use the equation MCΔT to find the energy change. The specific heat will be 1 and we just have to measure the water to find the mass and take the temperature before and after we start to find the energy change.…