01/21/11
LAB EXERCISE I
After the first exercise : Simulating Simple diffusion
Which Materials diffused from the left beaker to the right beaker?
NaCl , Urea , Glucose
Which did not ?
Albumin
Why ?
Albumin’s Composition or charge was too great to diffuse through the membrane.
After the 2nd activity : Simulating Dialysis
What happens to the urea concentration in the left beaker (the Patient)?
It diffused to the right beaker
Why does this occur?
The excess amount must be diffused to reach equilibrium.
3rd exercise :Facilitated Diffusion
At ta fiven concentration, how does the amount of time it takes to reach equilibrium change with the number of carriers used to “build” the membrane?
The time needed decreases
Does the Diffusion rate of Na/Cl change with the number of receptors? Yes
What is the mechanism of the Na/Cl transport? The selective plasma membrane
If you put the same amount of glucose in the right beaker as in the left, would you be able to observe any diffusion? No they would be equal
Does being unable to observe diffusion necessarily mean that diffusion is not taking place? No the rate of diffusion may be either very rapid or gradual.
4th : Osmosis
Did you observe any pressure changes during this experiment ? if so in which beakers and which membranes?
I did not notice any pressure changes.
Why? Both of the pressures in the beakers were not equal.
Did the Na/Cl diffuse from the left beaker to the right beaker ? if so which memebranes?
The was no diffusion in the beakers
Why ? because the pressure was not equal , diffusion could not occur
Explain the relationship between solute concentration and osmotic pressure.
Higher concentration higher pressure
Does diffusion allow osmotic pressure to be generated?
No, there’s no effect.
Would pressure be generated if solute concentrations were equal on both sides of the membrane? No, the pressure would be stable
Why or why not? It would be stabe