Physioex 8.0 Experiment 1
Lab Report One
Exercise Five
Activity One
“Simulating Simple Diffusion”
Activity Two
“Simulating Facilitated Diffusion”
Jessica Ogola
Anatomy and Physiology 2401
Dr. Denyse Jones
02/02/2012
Objective:
The objective of this experiment is to perform the simulation of the movement of solutes from a higher concentration to a lower concentration within a given amount of time.
The goal of the exercise is to provide a simulation for the process of the facilitated diffusion of glucose through a selectively permeable membrane.
Introduction:
Experiment #1 and #2 are meant to show the processes of Simple and Facilitated diffusion. Both the solutes and water were used to show how they diffuse through a semi permeable membrane. In experiment #1 the process of using the solute and deionized water shows how much of the solute is passed through the membrane. In experiment #2 the rate of facilitated diffusion relied on the amount of carrier proteins available to transport the solute molecules.
Hypothesis – Activity 1: Simulating Simple Diffusion
“I hypothesize that all of the solutes will have some rate of diffusion through the permeable membrane.” Hypothesis – Activity 2: Simulating Facilitated Diffusion
“I hypothesize that a higher concentration gradient would result in a higher rate of glucose transport”
Materials and Methods:
Activity #1 Materials: * 2 Beakers * 4 MWCO Membranes * 20, 50, 100, 200 * 9.00 mM of each solute: * NaCl, Urea, Albumin, Glucose * Deionized water
Methods:
* The solutes NaCl, Urea, Albumin, and Glucose each in the amount of 9.00 mM were used respectively. * The first step is to place the appropriate membrane in between the beakers. * Dispense the appropriate solute (L) and Deionized water (R) into the beakers. * Set a timer for sixty minutes. When the time is finished read and record the data. * These steps are repeated for each solute and each additional
Exercise Five
Activity One
“Simulating Simple Diffusion”
Activity Two
“Simulating Facilitated Diffusion”
Jessica Ogola
Anatomy and Physiology 2401
Dr. Denyse Jones
02/02/2012
Objective:
The objective of this experiment is to perform the simulation of the movement of solutes from a higher concentration to a lower concentration within a given amount of time.
The goal of the exercise is to provide a simulation for the process of the facilitated diffusion of glucose through a selectively permeable membrane.
Introduction:
Experiment #1 and #2 are meant to show the processes of Simple and Facilitated diffusion. Both the solutes and water were used to show how they diffuse through a semi permeable membrane. In experiment #1 the process of using the solute and deionized water shows how much of the solute is passed through the membrane. In experiment #2 the rate of facilitated diffusion relied on the amount of carrier proteins available to transport the solute molecules.
Hypothesis – Activity 1: Simulating Simple Diffusion
“I hypothesize that all of the solutes will have some rate of diffusion through the permeable membrane.” Hypothesis – Activity 2: Simulating Facilitated Diffusion
“I hypothesize that a higher concentration gradient would result in a higher rate of glucose transport”
Materials and Methods:
Activity #1 Materials: * 2 Beakers * 4 MWCO Membranes * 20, 50, 100, 200 * 9.00 mM of each solute: * NaCl, Urea, Albumin, Glucose * Deionized water
Methods:
* The solutes NaCl, Urea, Albumin, and Glucose each in the amount of 9.00 mM were used respectively. * The first step is to place the appropriate membrane in between the beakers. * Dispense the appropriate solute (L) and Deionized water (R) into the beakers. * Set a timer for sixty minutes. When the time is finished read and record the data. * These steps are repeated for each solute and each additional
References: Human Anatomy and Physiology Laboratory Manual by Elaine N. Marieb and Susan Mitchell PhysioEx 8.0 Laboratory Experiments in Physiology http://www.biology-questions-and-answers.com/cell-membrane.html