Preview

presence of tannins in gooseberry leaves

Good Essays
Open Document
Open Document
329 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
presence of tannins in gooseberry leaves
CHAPTER 4
Presentation, Analysis and Interpretation of Data This chapter includes discussion on the presentation, analysis and interpretation of data obtained in the physical, chemical, and biological test.
1. Extraction and Isolation of Tannins
An extraction and isolation was conducted by the researchers to determine and calculate the amount of tannins from the Otaheite Gooseberry leaves. The results are the following:
Table 1
Result of the Extraction and Isolation of Tannins
Weight of the sample 170.51 g
Weight of evaporation dish 45.40 g
Weight of the Evaporating dish + extract 73.75 g
% yield weight of Tannins 16.63 %

The isolated Tannins from Otaheite Gooseberry leaves obtained a percentage yield of 16.63%coming from the weight of the sample which is 170.51 g.
2. Physical Properties of Tannins
2.1 Organoleptic Test
An organoleptic test was conducted by the researchers to determine the appearance, color, and odor of tannins from Otaheite Gooseberry leaves.
Tables 2
Result of the Organoleptic Test
Appearance Viscous
Color Dark brown
Odor Unpleasant

The table 2 shows the result of the organolepitc test conducted using Otaheite Gooseberry leaves wherein, the appearance is viscous, with a unpleasant odor and a dark brown color.
2.2 Solubility Test
An solubility test was conducted by the researchers to determine the solvent wherein the tannins can be best dissolved using Distilled water, Alcohol, Ether and Acetone.
Table 3
Results of Solubility test
Distilled water Soluble
Alcohol Insoluble
Ether Insoluble
Acetone Insoluble

The table 3 shows the result of solubility test conducted using Otaheitte Gooseberry leaves. Tannins are soluble in water and insoluble in alcohol, ether and acetone (organic solvents).
2.3 Melting Point
3. Chemical Properties of Tannins Expected Actual
a). Ferric chloride Brownish green (+) Brownish green
b). Gelatin Formation of precipitate (+) Green precipitate
c). Bromine Brownish orange (+)

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Solubility Lab

    • 653 Words
    • 3 Pages

    Part B. Solubility of Different Alcohols. Use your observations to complete the following table, rating each system as soluble, insoluble, or partially soluble. Alcohol Methyl alcohol Water Hexane…

    • 653 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    The Spanish crowns encouragement for colonists in central Mexico to intermarry with Indians in the early 1500’s created an intersectional experience for the first mestizo generation. This experience was created through a strategic process of plotting, rationalizing, and execution by the hands of the government, church and military. Post conquest, the government (Spanish Crowns) placed together a plan that manipulated race into setting up a hierarchical order that could either prohibit you or enable you economic and social privileges.…

    • 799 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Methodology: Using auto-pipette (20-100 microlitre), pipetted 0.06 mg of Taxifolin was added to 10ml conical flask and made up to the mark (10ml) with H2O.This procedure was repeated with the same amounts foe silymarin stock solution and silybum marianum extract. Taxifolin was transferred to 2ml brown bottle for HPLC auto sampler, this was also done to silymarin stock solution. Silybum marianum extract was filtered via a syringe filter (0.2 microlitre).The three samples were tested for concentrations of taxifolin via HPLC.This process was repeated a further 20 times with different amounts of taxifolin, silymarin stock solution and silybum marianum extract. Results of Taxifolin standard shown in table 1 and figure 1.…

    • 818 Words
    • 4 Pages
    Good Essays
  • Better Essays

    David Noviski CHM 244 B Dr. Bauman Isuru Wanigasooriyage Rachel Oliverio 11/1/16 Analysis of Anthocyanins and Anthocyanidins in Blackberries, Raspberries, and Blueberries Abstract The purpose of this experiment was to isolate anthocyanins, decompose them into their components (anthocyanidins), and to analyze the anthocyanins, and their corresponding anthocyanidins using paper chromatography, and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g, followed by blackberries at 1.19 x 10-6 g, finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins, since it contained the most streaks on the chromatography paper. The main conclusion that can be drawn from this experiment is that blueberries contain the most anthocyanins per gram compared to raspberries and blackberries.…

    • 1868 Words
    • 8 Pages
    Better Essays
  • Satisfactory Essays

    Gerard Jones, in “Violent Media is Good for Kids,” expresses personal experiences about how violent comic books can often help children break through their shells of self-isolation. Because Jones largely discusses his own opinions, I feel the essay should provide more statistical evidence to support his thoughts. Although I agree with his point that children do need to have an outlet to express their underlying rage, I think a boundary of how extreme we can allow children to stretch this idea needs to be set.…

    • 934 Words
    • 4 Pages
    Satisfactory Essays
  • Better Essays

    Nitric Oxide

    • 2102 Words
    • 9 Pages

    Goosefoot, or Chenopodium ambrosioides, is a tall herb native to Central and South America, where it's also known as epazote. Traditional healers use the leaves, roots and flowers to treat colds, leishmaniasis, menstrual disorders, uterine fibroids and uterine hemorrhaging. The plant is rich in flavonoids, terpenes and steroids and has potent antioxidant and anticancer action. A study published in the April…

    • 2102 Words
    • 9 Pages
    Better Essays
  • Better Essays

    and pulp ethanolic extract. Department of Microbiology Faculty of Pharmacy and BiochemistryUniversity of Zagreb in conjunction with the Croatia and Department of Pharmacognosy Faculty of Pharmacy and Biochemistry University of Zagreb, Croatia. 2004.…

    • 1275 Words
    • 6 Pages
    Better Essays
  • Satisfactory Essays

    Maturity effects: Maturity at harvest had a marked effect on ORAC, total anthocyanins and total phenolics of the berries, for the "Brightwell" and "Tifblue" cultivars of rabbiteye blueberries which were the only two cultivars evaluated. Berries harvested immediately after turning blue had lower ORAC and total anthocyanins than berries well matured that were harvested 49 days later. ORAC and total anthocyanins increased 224% and 261% respectively, in the "Brightwell" cultivar, while in "Tifblue" they increased 164% and 176% respectively, with increasing maturity. Total phenolics increased by 169% and 113% in the "Brightwell" and "Tifblue" cultivars, respectively, with increased…

    • 99 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    Elderberry Fever

    • 479 Words
    • 2 Pages

    A study conducted by Krawitz and colleagues in 2011 where the black elderberry was seen as a beneficial herbal supplement for treating influenza and bacterial infection through the enhancement of cytokine production and T cell activation. Sambucus nigra contains anthocyanins, which is a bioactive compound that responsible for colors such as red, purple, or blue. In addition, it also provides various health benefits. Studies of anthocyanins for animal and in vitro reduced necrotic and apoptotic cell death and lower the infarct risks through anti-inflammatory and relaxant effects on coronary arteries (Krawitz et al., 2011). Krawitz and colleagues (2011) conducted a study to find out if a standardized elderberry liquid extract wo against bacterial…

    • 479 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    The objective of this experiment was to resolve a given sample into its components by paper chromatography, to evaluate the retention factor of each component in the sample and to compare the relative solubility of the various components of a mixture of colors in a given solvent.…

    • 571 Words
    • 3 Pages
    Better Essays
  • Satisfactory Essays

    Herbal medicine is oldest and the most common system used worldwide but now it is less recognized because of the advancement of technology in this modern world, people usually use laboratory produced medicines which are common in drug stores the reason that made herbal medicines the least prioritized though it is obviously hundred percent organic compared to laboratory produced medicines. Herbal medicines were commonly used in the traditional times but now, people want the herbal medicines to be prepared easily, to be taken up easily without such procedures and to be tasted with different fruit flavors. With this, the researchers came up with the idea to make a study to prove that herbal medicines are more reliable and effective than laboratory produced medicines.…

    • 425 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    V. Macrocarpon Case Study

    • 941 Words
    • 4 Pages

    macrocarpon which inhibited by 35 % cell growth of SW620 but not SW480. By other hand, an enriched extract in anthocyanins obtained from V. myrtillus Berry inhibited by 7 % at 200 mg/mL HT29 cell growth and, by 34 % and 3 % in HCT116 at 4 mg/mL and 2 mg/mL, respectively (Zhao and others 2004; Katsube and others 2003). Thereafter, it was reported that an enriched fraction of anthocyanins from V. ashei readeberry was more cytotoxic (IC50 between 15 – 50 µg/mL) against CaCo-2 and HT29 cells than enriched fractions in tannins (IC50 between 50- 100 µg/mL) or phenolic acids (IC50 = 1 mg/mL) (McDougall and others 2008; Maldonado and Raul 2010).…

    • 941 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    C Nardus Leaves Lab Report

    • 3038 Words
    • 13 Pages

    Saponins, alkaloids, tannins, flavonoids and Phenolic compounds were qualitatively analyzed. The summary of phytochemical analysis results of aqueous crude extracts of C. nardus leaves was tabulated in table 4.1.…

    • 3038 Words
    • 13 Pages
    Powerful Essays
  • Good Essays

    T. Conophorum Lab Report

    • 920 Words
    • 4 Pages

    conophorum leaves contains saponins, tannin, steroid, flavonoid, terpenoids and cardiac glycosides as their major secondary metabolites while alkaloid, phlobatannin and anthraquninone were absent (Table 4). This result was contrary to the report of Ayoolaet al.[33] who reported presence of alkaloid, tannin and absence of saponins, steroid, flavonoid, terpenoids, cardiac glycosides, phlobatannin and anthraquninone for T. conophorum seeds while some of the phytochemical constituents reported in our study were similar to the work of Obianime and Uche[34] who reported the presence of flavonoid, tannin, alkaloids, carbohydrate, volatile oils, terpenoids, saponins and cardiac glycosides in the seeds of Tetracarpidium conophorum. Also, the extract contains high content of total phenolic (29.31 ± 0.47) and vitamin C (23.49 ± 0.04) with appreciable amount of total flavonoid (10.32 ±…

    • 920 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    In this extraction 100grams of plant powdered root was suspended in 1000 ml methanol and shake it well and keep it for 15 days. This type of the extraction was collected and dried the extraction by removing solvent from distillation under reduced pressure.…

    • 1681 Words
    • 7 Pages
    Powerful Essays

Related Topics