Primary Cell Culture Guide
ATCC® PRIMARY CELL Culture Guide tips and techniques for culturing primary cells
The Essentials of
Life Science Research
Globally Delivered™
Table of Contents
ATCC® Primary Cell Solutions®...........................................................................1
ATCC Primary Human Endothelial Cell Solutions.............................................5
ATCC Primary Human Smooth Muscle Cell Solutions.......................................9
ATCC Primary Human Epithelial Cell Solutions...............................................12
ATCC Primary Human Fibroblast Solutions.....................................................15
ATCC Primary Human Keratinocyte Solutions................................................19
ATCC Primary Human Melanocyte Solutions..................................................22
ATCC Human Mesenchymal Stem Cell and Differentiation Solutions..........25
References .......................................................................................................31
www.atcc.org
ATCC® PRIMARY CELL SOLUTIONS®
Guide to Culturing Human Primary Cells
Primary cells and cell types
Primary cell cultures more closely mimic the physiological state of cells in vivo and generate more relevant data representing living systems. Primary cultures consist of cells that have been freshly derived from a living organism and are maintained for growth in vitro. Primary cells can be categorized according to the genus from which they are isolated, as well as by species or tissue type. Each mammalian tissue type is derived from the embryonic germ layer consisting of ectoderm, endoderm and mesoderm, which differentiate into the many cell types that organize into tertiary structures such as skin, muscle, internal organs, bone and cartilage, the nervous system, blood and blood vessels1. The cell types most frequently found in primary cell culture are epithelial cells, fibroblasts, keratinocytes, melanocytes, endothelial cells, muscle cells, hematopoietic
The Essentials of
Life Science Research
Globally Delivered™
Table of Contents
ATCC® Primary Cell Solutions®...........................................................................1
ATCC Primary Human Endothelial Cell Solutions.............................................5
ATCC Primary Human Smooth Muscle Cell Solutions.......................................9
ATCC Primary Human Epithelial Cell Solutions...............................................12
ATCC Primary Human Fibroblast Solutions.....................................................15
ATCC Primary Human Keratinocyte Solutions................................................19
ATCC Primary Human Melanocyte Solutions..................................................22
ATCC Human Mesenchymal Stem Cell and Differentiation Solutions..........25
References .......................................................................................................31
www.atcc.org
ATCC® PRIMARY CELL SOLUTIONS®
Guide to Culturing Human Primary Cells
Primary cells and cell types
Primary cell cultures more closely mimic the physiological state of cells in vivo and generate more relevant data representing living systems. Primary cultures consist of cells that have been freshly derived from a living organism and are maintained for growth in vitro. Primary cells can be categorized according to the genus from which they are isolated, as well as by species or tissue type. Each mammalian tissue type is derived from the embryonic germ layer consisting of ectoderm, endoderm and mesoderm, which differentiate into the many cell types that organize into tertiary structures such as skin, muscle, internal organs, bone and cartilage, the nervous system, blood and blood vessels1. The cell types most frequently found in primary cell culture are epithelial cells, fibroblasts, keratinocytes, melanocytes, endothelial cells, muscle cells, hematopoietic
References: Tissue Eng. 7(2):211-228), 2001. 3. Zuk PA, et al. Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell. 13:42794295, 2002. 6. Kang SK, et al. Neurogenesis of Rhesus adipose stromal cells. J. Cell Sci. 117:4289-4299, 2004. 7. Safford KM, et al. Stem cell therepy for neurologic disorders: Therapeutic potential of adiposederived stem cells. Current Drug Targets 6:57-62, 2005. 14. Freshney, RI. Culture of Animal Cells: A Manual of Basic Technique, 6th Ed. John Wiley & Sons, Hoboken, New Jersey, 2010. 26, 2012. 17. Rieger MM. Keratinocyte function and skin health. Cosmetics and Toiletries, July 01, 1992. Downloaded from the WWW on March 26, 2012 from Access My Library at http://www.