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Production Of Lyco Cookies

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Production Of Lyco Cookies
Optimization of extraction of lycopene for production of lyco-cookies
Short running title : PRODUCTION OF LYCO-COOKIES

Keywords : antioxidant, carotenoids, n-hexane, cookies, supplement

Meena Vankudre*, Vina D. L. Putra and Vaibhav Zamare

Department of Biotechnology, Sinhgad College of Science, Affiliated to University of Pune,
Ambegaon (BK), Pune 411041, India
*Author for correspondence e-mail : meenahv@gmail.com

Abstract
In today’s world, people tend to be exposed to unhealthy lifestyle. This includes their diet, habitual activities, and environment which continuously affect their stress level, which may lead to development of various diseases. Lycopene is a bright red carotenoid which is present abundantly in tomatoes and other bright colored fruits and vegetables. It, as a natural source of antioxidant, has been demanded due to its biological and physiochemical properties. Therefore, there is a need to develop the form of Lycopene supplement. Lycopene was extracted from tomato wastes by using acetone and purified by column chromatography. The extract was characterized by UV-Vis spectrophotometer. An average recovery of 40 mg lycopene / kg tomato wastes was obtained. Lyco-cookies were prepared by using common ingredients containing 3 mg lycopene per serving.

Introduction Lycopene is an open chain pigment of unsaturated carotenoid which is responsible for red colour found in tomatoes, grapes, watermelon, and other bright colored fruits and vegetables. Its function in plants is to absorb light in photosynthesis and to protect plants from the excess of light1. Lycopene is an acyclic isomer of β-carotene with 11 conjugated double bonds, normally in the all-trans configuration. Because its double bonds are conjugated, lycopene absorbs radiation at long wavelength of the visible spectrum2.
Lycopene has been characterized as a strong antioxidant, implicated in reduced incidence of cardiovascular disease and reduced frequency of several types



References: 1. Amin Ismail, Kin-Weng Kong, & Hock-Eng Khoo, Revealing the Power of the Natural Red Pigment Lycopene, Molecules, 2010, 15, 959-987 2 3. E. Giovannucci, E. B. Rimm, Y. Liu, M. J. Stampfer, & W. C. Willett, A prospective study of tomato products, lycopene, and prostate cancer risk, J Nat, l Cancer Inst 94 (2002) 391-398. 4. R. B. van Breemen & N. Pajkovic, Multitargeted Therapy of Cancer By Lycopene, Cancer Lett. 2008. 269(2). 5. D.M.F. Salvadori & C. Scolastici, Lycopene activity against chemically induced DNA damage in Chinese hamster ovary cells, Toxicology in Vitro 21 (2007) 840–845 6 8. Cox, S. E., Stushnoff, C. and Sampson, D. A. 2003. Relationship of fruit color and light exposure to lycopene content and antioxidant properties of tomato. Can. J. Plant Sci. 83: 913–919. 9. W. Stahl and H. Sies, Lycopene-rich products and dietary photoprotection, Photochem.Photobiol. Sci., 5, (2006), 238-242 10 11. FAO/WHO, 2006. Evaluation of certain food additives (Sixty-seventh report of the Joint FAO/WHO Expert Committee on Food Additives). Geneva, World Health Organization (WHO) Technical Report Series, No. 940. 12. Aghel N, Ramezani Z, and Amirfakhrian S, Isolation and Quantification of Lycopene from Tomato Cultivated in Dezfoul, Iran, JJNPP, 6(1), (2011) 9-15 13 14. Liana Maria Alda, S.Alda and L. Niţă, Lycopene content of tomatoes and tomato products, Journal of Agroalimentary Processes and Technologies (2009), 15 (4), 540-542. 15. Michelle Lamke et. al, Butter Balls, We Energies Cookie Boom (2011), 19. 16. David Peryam, The 9-Point Hedonic Scale, Peryam and Kroll Research Corporation, (1998), 4.1-4.13. 17. David Peryam & Norman Girardot, Advance in taste-test Method, Peryam and Kroll research corporation, (1998), 2.1-2.11 18 19. David Kilcast , Sensory analysis for food and beverage quality control: A Practical Guide, Woodhead Publishing Limited, (2010), 51-64.

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