Prokaryotes Lab Report
The prokaryotes
Student name : Lina Qasem AL-hyasat .
Engineer : Ahmad Shrab .
Introduction
This lab is purposed to familiarize basic equipment and techniques used in the study of microorganisms. In addition, learn some basic techniques used in identifying prokaryotes and make and view microscope slides of some common prokaryotes. In this lab , I worked two experiments , the first one is cultivation bacteria "colony ", and When microorganisms are cultivated in the laboratory, a growth environment called a medium is used , this medium should be sterile ,When grown and isolated by placing onto medium that known as inoculation .The purpose from this …show more content…
-waited even dryed.
-Added one drop of oil immersion.
-put the slide on the stage of compound microcope.
-finaly,watched the slide.
Isolation of bacteria method
-wiped working area with disinfectant.
-Obtained a sterile petri dish of nutrient agar,kept led on ,and used the marker to divided the petri dish ,into teo sector.
-opened lte lid slightly,inoculate sector number 1 with thumbprint. -Taked sterial wire loop to transferred colony from a broth to an agar plate.
-The loop should be cooled in the air for 10 to 20 sec and placed on the line drawed.
-Remove a sample from a broth culture by using a sterile wire loop.
-Transferred the sample from borth to agar plat.
- When inoculated an agar plate, drawed the loop very lightly over the surface while being careful not to break the surface. A zig-zag motion used.
-The name wrote on the plate ,then placed it's into incubator in a 37 degree incubator for 48 hours.
Result of Gram stain:
Result of Isolation of bacteria in the following pictures :
Disscusion :
Microorganism are organism that are too small and cannot be seen with naked
Student name : Lina Qasem AL-hyasat .
Engineer : Ahmad Shrab .
Introduction
This lab is purposed to familiarize basic equipment and techniques used in the study of microorganisms. In addition, learn some basic techniques used in identifying prokaryotes and make and view microscope slides of some common prokaryotes. In this lab , I worked two experiments , the first one is cultivation bacteria "colony ", and When microorganisms are cultivated in the laboratory, a growth environment called a medium is used , this medium should be sterile ,When grown and isolated by placing onto medium that known as inoculation .The purpose from this …show more content…
-waited even dryed.
-Added one drop of oil immersion.
-put the slide on the stage of compound microcope.
-finaly,watched the slide.
Isolation of bacteria method
-wiped working area with disinfectant.
-Obtained a sterile petri dish of nutrient agar,kept led on ,and used the marker to divided the petri dish ,into teo sector.
-opened lte lid slightly,inoculate sector number 1 with thumbprint. -Taked sterial wire loop to transferred colony from a broth to an agar plate.
-The loop should be cooled in the air for 10 to 20 sec and placed on the line drawed.
-Remove a sample from a broth culture by using a sterile wire loop.
-Transferred the sample from borth to agar plat.
- When inoculated an agar plate, drawed the loop very lightly over the surface while being careful not to break the surface. A zig-zag motion used.
-The name wrote on the plate ,then placed it's into incubator in a 37 degree incubator for 48 hours.
Result of Gram stain:
Result of Isolation of bacteria in the following pictures :
Disscusion :
Microorganism are organism that are too small and cannot be seen with naked