Protease Production Case Study
3.2. Screening and optimization of the most effective factors on protease production by S. marasensis
3.2.1. One-facto-at-a-time
Since protease production and growth of S. marasensis SR-081 Halo was affected by nutritional factors and culture conditions, the medium was initially optimized by one-factor-at-a-time method. Maximum growth of the strain and protease production was observed at pH 7‒8 (Fig. 2a and 2b), 40 ºC (Fig. 2c and 2d), and inoculum size of 10% (v/v) (Fig. S5a), that to reduce the impact of inoculum medium on composition of protease production medium and prevention of a rapid drop of protease production, inoculum volume of 2% was selected. The optimum salt requirement for growth and protease production (Fig 1b, 1c, and 1d) was …show more content…
marasensis were found to be NaCl 200 g L−1, glucose 12.5 g L−1, peptone 3.0 g L−1, MgCl2.6H2O 5.5 g L−1, CaCl2 2.8 g L−1, MnSO4 1.5 g L−1, K2SO4 3.0 g L−1, KNO3 5.5 g L−1, pH 7, and inoculum 2% at 40 °C and about 3.4 fold (from 6 to 20.26) enhancement of protease production has been achieved by optimizing the conditions.
Lately enzyme media optimization has been widely reported. Protease activity from Lysinibacillus fusiformis AU01 in presence of trisodium citrate 2.5 g L−1, yeast extract 3.6 g L−1, pH 7.9, and at the optimal temperature of 40 °C was enhanced 6 fold using Plackett-Burman and Central Composite statistical designs [31]. Rai et al. [32] have also reported a new alkaline serine protease from Bacillus subtilis DM-04 with highest activity at 45 °C, 72 h of incubation and 1.25% (w/v) of beef extract as the most effective factors on protease production.
In order to verify the model adequacy, optimum conditions for protease yield was performed and the maximum response of protease production was 19.93 U mL−1. The result confirmed the optimization of protease production by response surface methodology. Thus, the optimization of protease secreted by S. marasesis strain SR-081 Halo was successfully developed by FFD and
3.2.1. One-facto-at-a-time
Since protease production and growth of S. marasensis SR-081 Halo was affected by nutritional factors and culture conditions, the medium was initially optimized by one-factor-at-a-time method. Maximum growth of the strain and protease production was observed at pH 7‒8 (Fig. 2a and 2b), 40 ºC (Fig. 2c and 2d), and inoculum size of 10% (v/v) (Fig. S5a), that to reduce the impact of inoculum medium on composition of protease production medium and prevention of a rapid drop of protease production, inoculum volume of 2% was selected. The optimum salt requirement for growth and protease production (Fig 1b, 1c, and 1d) was …show more content…
marasensis were found to be NaCl 200 g L−1, glucose 12.5 g L−1, peptone 3.0 g L−1, MgCl2.6H2O 5.5 g L−1, CaCl2 2.8 g L−1, MnSO4 1.5 g L−1, K2SO4 3.0 g L−1, KNO3 5.5 g L−1, pH 7, and inoculum 2% at 40 °C and about 3.4 fold (from 6 to 20.26) enhancement of protease production has been achieved by optimizing the conditions.
Lately enzyme media optimization has been widely reported. Protease activity from Lysinibacillus fusiformis AU01 in presence of trisodium citrate 2.5 g L−1, yeast extract 3.6 g L−1, pH 7.9, and at the optimal temperature of 40 °C was enhanced 6 fold using Plackett-Burman and Central Composite statistical designs [31]. Rai et al. [32] have also reported a new alkaline serine protease from Bacillus subtilis DM-04 with highest activity at 45 °C, 72 h of incubation and 1.25% (w/v) of beef extract as the most effective factors on protease production.
In order to verify the model adequacy, optimum conditions for protease yield was performed and the maximum response of protease production was 19.93 U mL−1. The result confirmed the optimization of protease production by response surface methodology. Thus, the optimization of protease secreted by S. marasesis strain SR-081 Halo was successfully developed by FFD and