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Protein Hydrolysis and Characterization

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Protein Hydrolysis and Characterization
Protein Hydrolysis and Characterization
Group 7
Niez, Robert Francis,
*Orbin, Alfonso Ricardo*
Parro, Athena Emmanuelle Peralta, Christian
Department of Biological Sciences, University of Santo Tomas, Manila, Philippines

• Abstract Hydrolyzed Protein is protein that has been hydrolyzed or broken down into its component amino acids. While there are many means of achieving this, two of the most common are prolonged boiling in a strong acid (acid-HVP) or strong base or using an enzyme such as the pancreatic protease enzyme to stimulate the naturally-occurring hydrolytic process. A series of tests were performed and yielded positive results for each of the protein hydrolysis.
• Introduction
Proteins, from the Greek proteios, meaning first, are a class of organic compounds which are present in and vital to every living cell. In the form of skin, hair, callus, cartilage, muscles, tendons and ligaments, proteins hold together, protect, and provide structure to the body of a multicelled organism. In the form of enzymes, hormones, antibodies, and globulins, they catalyze, regulate, and protect the body chemistry. In the form of hemoglobin, myoglobin and various lipoproteins, they affect the transport of oxygen and other substances within an organism.
The total protein component of milk is composed of numerous specific proteins. The primary group of milk proteins are the caseins. There are 3 or 4 caseins in the milk of most species; the different caseins are distinct molecules but are similar in structure. All other proteins found in milk are grouped together under the name of whey proteins. The major whey proteins in cow milk are beta-lactoglobulin and alpha-lactalbumin. The major milk proteins, including the caseins, ß-lactoglobulin and a-lactalbumin, are synthesized in the mammary epithelial cells and are only produced by the mammary gland. The immunoglobulin and serum albumin in milk are not synthesized by the epithelial cells. Instead, they are



References: Voet D, Voet JG. (2004). Biochemistry Vol 1 3rd ed. Wiley Van Holde KE, Mathews CK. (1996). Biochemistry. Menlo Park, Calif: Benjamin/Cummings Pub. Co., Inc. Murray RF, Harper HW, Granner DK, Mayes PA, Rodwell VW. (2006). Harper 's Illustrated Biochemistry. Jones, Alicia Noelle (2002). "Density of Milk". The Physics Factbook. Greenwood, Norman N.; Earnshaw, A. (1997), Chemistry of the Elements (2nd ed.)

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