Pt1420 Unit 3 Data Analysis

This updated manuscript explains how they defined orthologous gene groups and the results of the assessment of the selection pressure on duplicated genes. The writing is clear and their conclusions are reasonable.

I'm almost satisfied with this revised manuscript. Now I can recommend this manuscript to publish in this journal. The followings are minor comments.

# Minor comments

p.11 l.12 '27 primer pairs' must be '25 prime pairs'

p.11 l.13 '11 pairs of single-copy' must be '10 paris'

p.12 l.18 'deviation of the two models'. Please explain "two models" explicitly.

p.13 About the calculation method of KaKs in A.halleri genes. The authors add extra codons when they found more than one variable sites in a codon. This 'concatenated codons method' increases the number of comparison and may cause underestiamtion. Please discuss on this issue.
This part is difficult to understand. I was barely to understand what they say with the help of Suppl Fig S3.

p.19 from l.20. About the method to determine the number of duplicated genes. This strategy is conservative and will underestiamte the copy number. Please discuss on this possibility and the possible effect on the conclusion.

p.21 l.23 Please explain what 'unclear selection'