Pure Cultures Lab Report
Jody Kaur
Pure Cultures Lab
1/31/13
Introduction : Pure cultures are made of only one type of organisms and can be used to study their properties. A method used to isolate pure cultures is making a steak-plate, which is a dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod, culture was taken from the tube and dragged across area 1 several time,of the agar. The agar was then turned 90º, and the loop was flamed and cooled. Taking some culture from area 1, it was dragged over area two,and the same steps were done for areas 3 and 4.Another technique used was spread-plate, where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod was dipped in 95%
Pure Cultures Lab
1/31/13
Introduction : Pure cultures are made of only one type of organisms and can be used to study their properties. A method used to isolate pure cultures is making a steak-plate, which is a dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod, culture was taken from the tube and dragged across area 1 several time,of the agar. The agar was then turned 90º, and the loop was flamed and cooled. Taking some culture from area 1, it was dragged over area two,and the same steps were done for areas 3 and 4.Another technique used was spread-plate, where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod was dipped in 95%