Reaction Rates of Barley Alpha Amylase with Starch at Specific Ph's
Reaction Rates of Barley Alpha Amylase with Starch at Specific pH 's and Temperatures
Page 1
Abstract Enzyme 's are used as catalysts in certain reactions. They help lower the activation energy needed for the reaction to go to completion. At optimum temperature and pH the amount of collisions of substrate and enzyme is at its highest, any deviation from the optimum temperature and pH will result in the denaturization of the enzyme. The purpose of this experiment is to find the optimum temperature and pH for the reaction of Barley alpha-amylase and starch. The predicted optimum temperature and pH is 50-60 degrees Celsius and pH of 5-6. To test this hypothesis, a starch solution was mixed with the amylase and then tested for absorbance, using a spectrophotometer, at several different temperatures and pHs. From these experiments it was concluded that the optimum temperature was 55 degrees Celsius and the optimum pH was 5. these results lead to the conclusion that the Barley seed is best suited to be in soil that is relatively acidic and warm.
Page 2
Introduction
The purpose of this enzyme kinetics lab was to experimentally find the optimum pH and temperature at which the enzyme, Barley alpha-amylase, would react with a starch solution. An enzyme is a special protein that is used to catalyze certain reactions (Campbell, 2005). A catalyst is a substance that speeds up a reaction without being consumed by the reaction (Campbell, 2005). Reactions are measured by their rate or reaction. The reaction rate can be increased and decreased based on the amount of activation energy is applied to it. The purpose of the enzyme is to lower this activation energy to create product more efficiently (Campbell, 2005). When an enzyme binds with a substrate it forms an enzyme-substrate complex. This complex is how the enzyme reacts with the substrate to form the product, such as when the barley alpha amylase bonds with the starch to form barley alpha amylase, maltose,
Page 1
Abstract Enzyme 's are used as catalysts in certain reactions. They help lower the activation energy needed for the reaction to go to completion. At optimum temperature and pH the amount of collisions of substrate and enzyme is at its highest, any deviation from the optimum temperature and pH will result in the denaturization of the enzyme. The purpose of this experiment is to find the optimum temperature and pH for the reaction of Barley alpha-amylase and starch. The predicted optimum temperature and pH is 50-60 degrees Celsius and pH of 5-6. To test this hypothesis, a starch solution was mixed with the amylase and then tested for absorbance, using a spectrophotometer, at several different temperatures and pHs. From these experiments it was concluded that the optimum temperature was 55 degrees Celsius and the optimum pH was 5. these results lead to the conclusion that the Barley seed is best suited to be in soil that is relatively acidic and warm.
Page 2
Introduction
The purpose of this enzyme kinetics lab was to experimentally find the optimum pH and temperature at which the enzyme, Barley alpha-amylase, would react with a starch solution. An enzyme is a special protein that is used to catalyze certain reactions (Campbell, 2005). A catalyst is a substance that speeds up a reaction without being consumed by the reaction (Campbell, 2005). Reactions are measured by their rate or reaction. The reaction rate can be increased and decreased based on the amount of activation energy is applied to it. The purpose of the enzyme is to lower this activation energy to create product more efficiently (Campbell, 2005). When an enzyme binds with a substrate it forms an enzyme-substrate complex. This complex is how the enzyme reacts with the substrate to form the product, such as when the barley alpha amylase bonds with the starch to form barley alpha amylase, maltose,
Cited: Campbell, N.A., and Reece, J.B. 2005. Biology, 7th ed. Benjamin/Cummings Publishing Co., San Francisco, California. Vliet, K.A. (ed.). 1996. A Laboratory Manual for Integrated Principles of Biology: Part One – BSC2010L. Ginn Press, Needham Heights, Massachusetts.