Rhubarb Epidermis Cells Lab Report
Plasmolysis in Rhubarb Epidermis Cells
Aim:
To see if rhubarb changes when it is placed in solutions of different concentrations.
Introduction:
"Plasmolysis in Rhubarb Epidermis Cells" is an experiment to see whether or not rhubarb changed its cell structure when placed within different types or solutions. "A single layer of plant cell is placed on a micrscope slide and either distilled water or 5% NaCl solution is added to the cells. Osmosis will occur resulting in either turgid cells or plasmolysed cells." (www.practicalbiology.org)
Materials: forceps (from rhubarb stem)
1 slide
1 coverslip dropping pipette
3 small pieces of filter paper
1 microscope access to 30% Na solution bottle tweezers Method:
1.A piece of …show more content…
red coloured forcep was peeled from the stem of the rhubarb and was placed onto a slide using tweezers.
2.The dropping pippette was then used to put a drop of water onto the forcep that was on the slide.
3.A cover slip was then carefully placed onto the drop of water and it was made sure that the forcep would not flip over.
4.After the slide was prepared, it was then put onto the microscope using x100 view. The microscope was adjusted so that it was zoomed on a piece of the forcep.
5.When a piece of the forcep was located through the eyepiece of the microscope at x100 view, the magnification was increased to x400 and was refocused using only the fine focus knob.
6.When focused, the image that was seen had to be recorded by drawing what was observed. The image below shows what was observed when it was focused at x100 and x400 magnification view. The cell wall and cytoplasm were labelled.
7.After the first observation and drawing was done, the slide was removed from the microscope and the pippette was used again to add another drop of substance onto the same slide, except the substance was a 30% salt solution.
8.The slide was placed back under the microscope and was observed with x100 magnification view. Focusing again on a piece of forcep, the microscope magnification increased back up to x400 and the fine focus knob was adjusted so that observing the piece of cell located was …show more content…
clear.
9.What was to be observed were cells becoming plasmolysed and to see how the structures of the cell became after the salt solution was added.
10."As soon as the cell membrane separates from the cell wall a cell is plasmolysed." When this happened, the object/cell that was observed was drawn and labelled. The cell wall, cell membrane, cytoplasm and nucleus were all labelled on the drawn diagram below.
Result:
The first observation with the water being placed onto a piece of rhubarb forcep showed that the cell was turgid meaning that water goes into the cell, which water did indeed go into the cell as the cytoplasm was circulating around the membrane with vacuoles in sight. The cell membrane was still closely attached near the cell wall.
The second observation with the salt solution replacing the water on the piece of rhubarb forcep showed that the cell was plasmolysed meaning that water exits the cell. During observation, the membrane shrank in size and away from the cell wall becoming smaller. It drifted off to one side and the red colour slowly
faded.
Conclusion:
Different concentrations of solutions affect the cells of the forcep. In the case of the experiment, when the salt solution was added to the plant, the water in the plant is the higher concentration, therefore osmosis occured and water moved out from the higher concentration to the lower concentration causing the cell to lose water and shrink. Since water exits the cell, the process is called plasmolysis. As for the water being added to the forcep, water continued flowing into the cell because it moved from the lower concentration(since pure water outside the cell is 100% pure water) area to a lower concentration area making a turgid process.
Reference: www.practicalbiology.org en.wikipedia.org/Advanced Microbiology and Human Biology Lab Workbook pg10
Aim:
To see if rhubarb changes when it is placed in solutions of different concentrations.
Introduction:
"Plasmolysis in Rhubarb Epidermis Cells" is an experiment to see whether or not rhubarb changed its cell structure when placed within different types or solutions. "A single layer of plant cell is placed on a micrscope slide and either distilled water or 5% NaCl solution is added to the cells. Osmosis will occur resulting in either turgid cells or plasmolysed cells." (www.practicalbiology.org)
Materials: forceps (from rhubarb stem)
1 slide
1 coverslip dropping pipette
3 small pieces of filter paper
1 microscope access to 30% Na solution bottle tweezers Method:
1.A piece of …show more content…
red coloured forcep was peeled from the stem of the rhubarb and was placed onto a slide using tweezers.
2.The dropping pippette was then used to put a drop of water onto the forcep that was on the slide.
3.A cover slip was then carefully placed onto the drop of water and it was made sure that the forcep would not flip over.
4.After the slide was prepared, it was then put onto the microscope using x100 view. The microscope was adjusted so that it was zoomed on a piece of the forcep.
5.When a piece of the forcep was located through the eyepiece of the microscope at x100 view, the magnification was increased to x400 and was refocused using only the fine focus knob.
6.When focused, the image that was seen had to be recorded by drawing what was observed. The image below shows what was observed when it was focused at x100 and x400 magnification view. The cell wall and cytoplasm were labelled.
7.After the first observation and drawing was done, the slide was removed from the microscope and the pippette was used again to add another drop of substance onto the same slide, except the substance was a 30% salt solution.
8.The slide was placed back under the microscope and was observed with x100 magnification view. Focusing again on a piece of forcep, the microscope magnification increased back up to x400 and the fine focus knob was adjusted so that observing the piece of cell located was …show more content…
clear.
9.What was to be observed were cells becoming plasmolysed and to see how the structures of the cell became after the salt solution was added.
10."As soon as the cell membrane separates from the cell wall a cell is plasmolysed." When this happened, the object/cell that was observed was drawn and labelled. The cell wall, cell membrane, cytoplasm and nucleus were all labelled on the drawn diagram below.
Result:
The first observation with the water being placed onto a piece of rhubarb forcep showed that the cell was turgid meaning that water goes into the cell, which water did indeed go into the cell as the cytoplasm was circulating around the membrane with vacuoles in sight. The cell membrane was still closely attached near the cell wall.
The second observation with the salt solution replacing the water on the piece of rhubarb forcep showed that the cell was plasmolysed meaning that water exits the cell. During observation, the membrane shrank in size and away from the cell wall becoming smaller. It drifted off to one side and the red colour slowly
faded.
Conclusion:
Different concentrations of solutions affect the cells of the forcep. In the case of the experiment, when the salt solution was added to the plant, the water in the plant is the higher concentration, therefore osmosis occured and water moved out from the higher concentration to the lower concentration causing the cell to lose water and shrink. Since water exits the cell, the process is called plasmolysis. As for the water being added to the forcep, water continued flowing into the cell because it moved from the lower concentration(since pure water outside the cell is 100% pure water) area to a lower concentration area making a turgid process.
Reference: www.practicalbiology.org en.wikipedia.org/Advanced Microbiology and Human Biology Lab Workbook pg10