Rivenburg jennifer SC121 section Unit 3
LABORATORY REPORT
Activity:
Blood Glucose Regulation
Name:
jennifer rivenburg
Instructor:
susan deno
Date:
12.01.2014
Predictions
Plasma glucose levels will be highest
1-3 hours after the meal
Plasma ketone levels will be highest before the meal (fasting)
Plasma insulin levels will be highest immediately after the meal (0 hr)
Plasma glucagon levels will be highest immediately after the meal (0 hr)
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables? i chose those because physical activity is something that can be controlled.
5. What type of assay was used to measure plasma glucose and ketone levels? the blood was taken and the plasma was removed from the sample and tested.
6. What type of assay was used to measure plasma insulin and glucagon levels? blood was taken and plasma was removed from the blood and then put in trays and tested
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.3
6.4
6.4
5.5
4.7
Glucose (mmol/l)
Sub. 2
Sub. 3
5.4
5.4
6.4
6.4
6.2
5.7
4.7
6.4
5.6
4.8
average
5.4
6.4
Sub. 1
100
94
6.3
5.6
4.7
58
67
73
Laboratory Report/ jennifer rivenburg/ Blood Glucose Regulation/ susan deno / 12.01.2014/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
116
119
96
90
72
71
60
63
76
66
average
112
93
64
71
66
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
0 hour post meal was the highest and 1 hour post meal was right behind it.
2. When was plasma
Activity:
Blood Glucose Regulation
Name:
jennifer rivenburg
Instructor:
susan deno
Date:
12.01.2014
Predictions
Plasma glucose levels will be highest
1-3 hours after the meal
Plasma ketone levels will be highest before the meal (fasting)
Plasma insulin levels will be highest immediately after the meal (0 hr)
Plasma glucagon levels will be highest immediately after the meal (0 hr)
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables? i chose those because physical activity is something that can be controlled.
5. What type of assay was used to measure plasma glucose and ketone levels? the blood was taken and the plasma was removed from the sample and tested.
6. What type of assay was used to measure plasma insulin and glucagon levels? blood was taken and plasma was removed from the blood and then put in trays and tested
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.3
6.4
6.4
5.5
4.7
Glucose (mmol/l)
Sub. 2
Sub. 3
5.4
5.4
6.4
6.4
6.2
5.7
4.7
6.4
5.6
4.8
average
5.4
6.4
Sub. 1
100
94
6.3
5.6
4.7
58
67
73
Laboratory Report/ jennifer rivenburg/ Blood Glucose Regulation/ susan deno / 12.01.2014/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
116
119
96
90
72
71
60
63
76
66
average
112
93
64
71
66
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
0 hour post meal was the highest and 1 hour post meal was right behind it.
2. When was plasma