Rm4 Synthesis Lab Report

The purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration, the substrate, on the enzyme, acid phosphatase, reaction. In an enzyme reaction, there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes, the majority of reactions would not occur because the activation energy level would be unattainable. With this in mind, the hypothesis was that the RM4 solution, the one with the most substrate, would have the greatest amount of product compared to the other four mixtures. The purpose of the second week experiment was to examine the effects of changing the pH