Electrophoresis: Using Electricity to Separate Molecules
Answer the following questions about the results of this activity. Record your answers in the boxes.
Send your completed lab report to your instructor. Don’t forget to save your lab report to your computer!
Activity 1 – Calibration
Record your data from Activity 1 in the boxes below. Enter the data (number of bands, description of the band patterns) you collected for the protein ladder in the appropriate columns for the gel concentrations (8%, 10%, 12%)
| | | | |
|Gel |8% |10% |12% |
| |4 |5 |6 |
|Number of Bands | | | |
| | |205 KDa |205 KDa |
|Description of Band |205 KDa |116 KDa |116 KDa |
|Patterns |116 KDa |67 KDa |67 KDa |
|(weights , relative sizes,|67 KDa |45 KDa |45 KDa |
|and spacing) |45 KDa |24 KDa |29 KDa |
| | | |14.2 KDa |
|