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Sclerotium Rolsfii Lab Report

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Sclerotium Rolsfii Lab Report
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Soil was found to be acidic in nature as pH was recorded in the range of 5.7±0.260 C. The mean temperature during sampling month varied from 27.8+0.830C. The moisture content of the soil was 17 ± 1 % during studies (Table-1).
From each rhizospheric soil, a single well distinct colony with different morphological characters was preferred for studies. On the basis of dissimilar morphological characters of the colonies, total twenty seven rhizobacterial isolates were isolated 7 from Coriander, 5 from Onion, 6 from Potato, 4 from Tomoto and 5 from Turmeric rhizospheric zones. Exponential growth was observed after 24 hrs of incubation. All organisms were found to be fast growers with generation time more 3.2±0.4 h-1.
To elucidate the rhizospheric
…show more content…
The rhizobacterial strains of plant Potato showed 83% of the potentiality in controlling growth of S. rolsfii followed by rhizobacterial strains of plant Onion 80 %,Tomoto plant 75%, Coriander plant 71 % and Turmeric plant only 20 % efficient in inhibiting growth of fungal strain. Maximum growth controlling effect towards S. rolsfii was observed in the rhizobacterial isolates of Potato and Onion isolates. When combined PGPR traits were studied it was recorded that highest IAA, Siderophore production and solubilization of phosphate was recorded in cor-4 followed by tur-3 rhizobacterial strain …show more content…
It is influenced by plant species (Hiltner, 1904). It is reported that the carbon assimilated by plants is releases 10–40% in the soil as a form of root secretions. These secretions are composed of numerous organic compounds that act as chemo-attractants for bacteria of rhizosphere even at very low concentration (Philippe, 2006). Communities of rhizobacteria recovered from rhizospheric zones of different plants in present study deplicated quite different phenotypic characters. It is also supported by the values of H’, which are greater for Coriander plant as compared to others. Similar findings are earlier also reported when diversity pattern from wheat, canola (Mittal and Johri, 2007) and Rice (Joshi et al., 2011) are studied. The rhizospheric structural diversity depends upon a array of factors such as age of plant, breed of cultivars, characters of soil, its pH, content of organic matter, availability of nutrient, root metabolites (Campbell, 1985) and traditional practices like tillage, irrigation, fertilization application, cropping, residue incorporation etc (Graysten et al.,

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