Serratiopeptidase Lab Report

Serratiopeptidase drug was purchased from MARS Therapeutics & Chemicals limited, Hyderabad, India. The standard drug Fenofibrate was purchased from USV limited, Himachal Pradesh, India. Assay kits for serum Total Cholesterol (TC), Triglycerides (TGL), High density lipoproteins were purchased from Erba Mannheim, Transasia Bio-Medicals limited, Himachal Pradesh, India. All other chemicals were of analytical grade.
The experiment was conducted using [12] male Albino Wistar rats (150-200g), at about 6-8 weeks of age. All the animals were procured from Sanzyme Ltd, Hyderabad, India. The animals were housed in poly acrylic cages (38cm x 23cm x 10cm) which have the capacity to carry six animals per cage. They were exposed to ambient temperature of
The effective doses of serratiopeptidase were fixed to be 10 mg/kg, b.w. and 50 mg/kg, b.w. orally. The solubility of serratiopeptidase was found to be in water and was insoluble in ether and alcohol.
Fenofibrate was considered to be as reference standard [13]. LD50 of fenofibrate (standard) was found to be >1242 mg/kg, p.o. the effective dose of fenofibrate was considered to be 65mg/kg, b.w, orally. The solubility of serratiopeptidase was found to be in 2:3 solutions of DMF and PBS (phosphate buffer solution) of pH-7.2.
Single dose of Triton X 100 (100mg/kg, b.w.) [13] was administered intraperitoneally to the groups II, III, IV, V. Normal controls were injected with saline alone. Triton X-100 was dissolved in freshly prepared, 0.9% sodium chloride immediately before administration to the rats and subsequently fed with atherogenic diet. When Triton X-100 reaches critical micelle concentration (CMC), in the range of 0.19 to 0.20Mm, [14] the cells undergo irreversible permeabilization of its membrane and structural collapse which results in progression of the disease. Physical parameters were estimated at 1, 7, 14, 21 days. Biochemical parameters were also estimated in the same intervals by collecting the blood samples from retro orbital plexus under mild ether anesthesia. Then the rats were sacrificed on 22nd day, the heart was dissected out and proximal aortas were isolated for histopathological studies.
Treatment with serratiopeptidase (test) at doses of 10 mg/kg and 50 mg/kg p.o. showed significant (p < 0.05) decrease in TC, LDL-C, VLDL-C, TGL, AI and significant (p < 0.05) increase in HDL-C. Treatment with standard drug fenofibrate also prevented the elevation of TC, LDL-C, VLDL-C, TGL, AI significantly (p < 0.05) and increased the HDL-C