Lady Macbeth and Macbeth have both shown guilt, but at different stages in the play. Isolating guilty feelings only begins to isolate them from the world around them. Macbeth is the first to feel guilt at the begging of the play, but towards the end he has nothing but isolation. Lady Macbeth has both isolation and guilt. In act III , scene two , lines 6 to 9, Lady Macbeth says, " Noughts had all's spent, where our desire is got without content. Tis safer to be that which we destroy". She is describing how the murder of Duncan has made them lose everything but has made them gain nothing. Her guilt has gotten the best of her by act IV, when all she has on her mind is guilt. When Lady Macbeth says in act V. scene two, line 43 to 44, "Heres the smell of blood still. All the perfumes of Arabia will not sweeten this little hand". She knows the murder is irrevocable, and nothing can be done to erase the deed from her mind.
Over ambitious feelings pave the way to isolation. When MacBeth becomes over ambitious about something. he begins to get absorbed into the ambition until eventually the rest of the world does not matter anymore. It seemed as though Lady Macbeth wanted the throne for her husband , more than he wanted it for himself.