Slide #1:
1. With the wooden applicator stick, dip the end into the blood and place a tiny drop onto slide #1 and put cover slip over it.
Slide#2
1. Place 1 drop of 0.9% NaCl with the drop of blood (use the filter paper to absorb excess liquid).
2. Add a cover-slip and use filter paper to absorb extra liquid (if necessary).
3. Observe cells under 40X then IOOX and then high power (400X).
4. The sheep blood in an isotonic solution
Slide#3
1. Place 1 drop of 10% NaCl solution onto the slide …show more content…
The sheep blood in a hypotonic solution
Results
In comparison to the first slide to the rest of the three slides, it can be concluded that the results were supportive of the hypothesis. The data collected from the second slide, it is shown that the cells were stable and didn’t experience much change. The data collected from the third slide shows that the cells was in a hypotonic solution. The data collected from the fourth slide shows the cells in a hypertonic solution.
Discussion
The evidence that was collected did not allow for the hypothesis to be rejected. The hypothesis was stated to be supportive based on the difference in all three slides. In the first method, a drop of sheep blood was placed on the slide and was covered with a coverslip. Second slide, the blood was placed on a slide then a solution of 0.9% NaCl was added. The cover slip was placed on the slide and was put under the microscope. After looking under the microscope, the cells were shown no different compared to the first slide. Third slide, the blood was placed on a slide then a solution of 10% NaCl was added. The cover slip was placed on the slide and was put under the microscope. After looking under the microscope, the cells were shown to look shriveled compared to the second slide. On the last slide, the blood was placed on a slide then a solution of dH20 was added. The cover slip was placed on the slide and was put under the microscope. After looking under the microscope, very little was shown on the slide as if the cells on the slide swell and burst. There was no error when the experiment took place knowing that the exact procedure was executed properly to lead to the results