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Simple Sugar Lab

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Simple Sugar Lab
Simple Sugars Lab

Emerson:
Hallie:

Background:
Benedicts solution is a chemical solution that changes color in the presence of glucose and other reducing sugars. It is a mixture of sodium or potassium citrate, sodium carbonate, and copper sulfate. It changes from its normal blue color to an orange, red, or yellow. it changes based on the amount of sugar used.
"Benedicts Solution." ­ Definition from Biology­Online.org. N.p., n.d. Web. 16 Jan. 2015. http://www.biology­online.org/dictionary/Benedicts_solution Infoplease. Infoplease, n.d. Web. 16 Jan. 2015. http://www.infoplease.com/encyclopedia/science/benedict­solution.html Question:
What common foods contain glucose? Materials:
­1% Milk
­ Skim Milk
­ Diet 7­up
­7­up
­ Sugar water
­ Honey
­Apple juice
­Low Calorie G­2
­ Peach tea
­ Diet Peach tea low calories
­Energy drink
­Lemonade flavored Spring Water No Calories
­Coconut flavored water with juice no calories
­Powerade
­Strawberry flavored water no calories
­Life water Flavor Fuji Apple pear no calories Procedure:
1. Create a data table with columns labeled Food substances, sugar prediction, observations, and results.
2. Read the food labels and predict the presence of simple sugar in each of the foods. record your prediction.
3. Prepare a hot water bath using a hot plate and 1000 mL beaker.

4. Label all test tubes. obtain a graduated cylinder. Add 10 mL of a different food substance to each test tube. then add 10 mL distilled water. swirl gently to mix.
5. Add 5 mL of Benedict's solution to each tube. use a clean stirring rod to mix the contents. 6. Using test tube holders, warm the test tubes in the hot water bath for 5 minutes. record your observations and results. Data Table:
Substance

Prediction

Color Before

Color After

Positive or
Negative

1% milk

Yes

cream blue

Blue/Yellow

Positive

Skim milk

No

light purple

Yellow/Green

Positive

Diet 7­up

Yes

light blue

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