Soaps Against E. Coli

1) TITLE (2pt): Testing antimicrobial props in soaps
2) INTRODUCTION (6 pts):
In this experiment we wanted to test how effective different types of soaps against E. Coli. For this experiment we were interested in looking at antimicrobial soaps vs. non-antibiotics soaps in their effectiveness to kill E. Coli. The protocol that we used was the Kirby Bauer technique. This technique was used to test the antimicrobial properties of each soaps. (“Kirby-Bauer” 2015).
3) METHODS (6 pts):
We first started by making up 6 different soap solutions. The dilution was 1:1. After that a positive control and negative control were made. The positive control was ampicillin. In order to do this we used the Kirby Bauer test((“Kirby-Bauer” 2015). Disk were then placed into the 8 different solutions to soak. Then we obtained two agar plate. We divided each of the plate into 4 different quadrants. We labeled each quadrant from 1-8. Then 0.4mL of E. Coli was then plated each agar plates. Then we put the different disks to their respective quarters. The plates then were placed into the incubator overnight at 37 degrees C. After 24 hours the zone of inhibition were measured (mm) for each of soaps.
4)
The range of zones of inhibition was from 13 to 37 mm. Shop Rite had the greatest zone of imbibition which was 37mm. The negative control, Lysol lil, method had no zone of inhibition they were overgrowth. Soft soap had the small zone of inhibition around the disk with