spme
EXPERIMENT 4
ANALYSIS OF HYDROCARBON IN COMMON FUELS BY SOLID-PHASE
MICROEXTRACTION (SPME) AND GAS CHROMATOGRAPHY-MASS
SPECTROMETRY (GC-MS)
Abstract
In the analysis the solid-phase microextraction (SPME) and capillary gas chromatography/mass spectrometry (GC/MS) was developed for the identification of volatile compounds (hydrocarbon) in fuel. The samples was used is (kerosene, diesel, thinner and petrol) and one unknown. After the analyte was extracted by SPME in 20min, it directly injected to the GCMS with desorption time 80sec.After the analysis was done, the result was stated at table 4.1, the unknown was identified as a petrol because the hydrocarbon presence in the unknown is the same with the hydrocarbon compound in the petrol sample. Aromatic hydrocarbon was presence in both sample petrol and unknown. Alkanes hydrocarbon was presence in other sample.
Objective
The objectives of this experiment are to perform sample preparation by SPME and to identify the components of hydrocarbon in common fuel using SPME-GC-MS.
Introduction
Solid phase microextraction, a simple, effective adsorption/desorption technique, eliminates the need for solvents or complicated apparatus for concentrating volatile or nonvolatile compounds in liquid samples or headspace. SPME is compatible with analyte separation/detection by gas chromatography or HPLC, and provides linear results for wide concentrations of analytes. By controlling the polarity and thickness of the coating on the fiber, maintaining consistent sampling time, and adjusting several other extraction parameters, an analyst can ensure highly consistent, quantifiable results from low concentrations of analytes.
Analyses of volatile or semivolatile organic environmental pollutants,flavor or fragrance components, and many other samples usually begin with concentrating the analytes of interest through liquid-liquid extraction, purge-and-trap, headspace, or various other
techniques.
ANALYSIS OF HYDROCARBON IN COMMON FUELS BY SOLID-PHASE
MICROEXTRACTION (SPME) AND GAS CHROMATOGRAPHY-MASS
SPECTROMETRY (GC-MS)
Abstract
In the analysis the solid-phase microextraction (SPME) and capillary gas chromatography/mass spectrometry (GC/MS) was developed for the identification of volatile compounds (hydrocarbon) in fuel. The samples was used is (kerosene, diesel, thinner and petrol) and one unknown. After the analyte was extracted by SPME in 20min, it directly injected to the GCMS with desorption time 80sec.After the analysis was done, the result was stated at table 4.1, the unknown was identified as a petrol because the hydrocarbon presence in the unknown is the same with the hydrocarbon compound in the petrol sample. Aromatic hydrocarbon was presence in both sample petrol and unknown. Alkanes hydrocarbon was presence in other sample.
Objective
The objectives of this experiment are to perform sample preparation by SPME and to identify the components of hydrocarbon in common fuel using SPME-GC-MS.
Introduction
Solid phase microextraction, a simple, effective adsorption/desorption technique, eliminates the need for solvents or complicated apparatus for concentrating volatile or nonvolatile compounds in liquid samples or headspace. SPME is compatible with analyte separation/detection by gas chromatography or HPLC, and provides linear results for wide concentrations of analytes. By controlling the polarity and thickness of the coating on the fiber, maintaining consistent sampling time, and adjusting several other extraction parameters, an analyst can ensure highly consistent, quantifiable results from low concentrations of analytes.
Analyses of volatile or semivolatile organic environmental pollutants,flavor or fragrance components, and many other samples usually begin with concentrating the analytes of interest through liquid-liquid extraction, purge-and-trap, headspace, or various other
techniques.
References: 1. http://www.sciencedirect.com/science 2. www.geocities.com/hpgc/chem700/spmenotes.pdf Appendix