Substrate Concentration Investigation

For a reaction to occur, you need a substrate (catechol). The reaction will occur faster if you have an enzyme. A reaction without an enzyme will still take place, but at a slower rate. If the substrate is mixed with a greater amount of distilled water, there is more enzymes needed in order for a reaction to occur; as the reaction is directly dependent on the enzymes. At low concentration of substrate, increasing the substrate concentration resulted in an increase rate. Above a certain level, the rate no longer changes when substrate concentration increased because the enzyme is saturated with substrate.
The rate of an enzyme-catalyzed reaction increases as the temperature is raised. If the temperature is too high, the enzyme will start to