Formatting:
Biological research papers should be written in Times New Roman font, size 11, and a line spacing of 1.5. On the upper right hand corner of each page should be your last name followed by the page number. Normal Page margins are advised.
The title of your paper …show more content…
They undergo four major stages of growth: a phase of enlargement, a phase of logarithmic multiplication, a stationary phase, and a phase of logarithmic death (Edick). These phases are also commonly known as phase I, II, III, IV respectively. Phase I is a time of protein synthesis and cell growth and during which there is no division taking place. DNA is first transcribed into RNA. Then mRNA molecules begin translation by attaching themselves to ribosomes. Meanwhile tRNA molecules with bases complementary to those of ribosome-attached mRNAs are dispersed in the cytoplasm. As individual tRNA molecules attach their mRNA sequence in the ribosome, they each bring with them an amino acid which is then added to the growing polypeptide chain through peptide bond formation. Then another tRNA molecule binds to the next corresponding codon and the process continues to repeat itself until a complete protein has been formed (Reece). By inhibiting peptide bond formation by binding to the 50s subunit of the ribosome Chloramphenicol effectively brings this process to a standstill and halts protein synthesis. When a critical number of proteins have formed in this manner, E Coli cultures enter growth phase II, the phase of logarithmic multiplication. During this phase bacteria undergo asexual binary fission. Growth is rapid at first while there is still an unlimited supply of nutrients and waste products have not yet begun to accumulate. As waste products accumulate and the nutrient supply begins to be used up, net growth halts as cell death becomes equal to cell formation. This stationary phase usually lasts for an extended period of time until finally nutrient supply falls below a critical point. The culture then enters a stage of logarithmic death which is the polar opposite of stage two. The number of live cells in the culture decreases slowly at first but