Superoxide Radical Scavenging Activity Lab Report

Figure 3 Superoxide radical scavenging activity. Values are mean ± SEM (n = 3). ABL, lyophilized aqueous extract of A. bilimbi fruits; ABP, petroleum ether fraction of ABL; ABE, ethyl acetate fraction of ABL; ABB, butanol fraction of ABL; ABW, aqueous fraction of ABL; QU, quercetin.

3.3.5 Nitric oxide radical scavenging activity
Lyophilized aqueous extract of Averrhoa bilimbi fruits (ABL) and its different solvent fractions exhibited a dose dependent inhibition of nitric oxide radicals. The IC50 values of ABL (71.75 ± 2.67 μg/ml), ABP (123.00 ± 4.58 μg/ml), ABE (61.50 ± 2.29 μg/ml), ABB (82.00 ± 3.05 μg/ml) and ABW (92.25 ± 3.43 μg/ml) were compared with the standard ascorbic acid (IC50 20.50 ± 0.76 μg/ml). Among all the fractions, ABE showed
Values are mean ± SEM (n = 3). ABL, lyophilized aqueous extract of A. bilimbi fruits; ABP, petroleum ether fraction of ABL; ABE, ethyl acetate fraction of ABL; ABB, butanol fraction of ABL; ABW, aqueous fraction of ABL.

3.3.8 Antiglycation activity
The antiglycation activity of plant extracts was assessed for their ability to inhibit advanced glycation end products (AGEs) formation in a BSA/glucose system. ABL, ABP, ABE, ABB and ABW possess potential antiglycation activity with IC50 values 51.25 ± 1.91 µg/ml, 92.25 ± 3.44 µg/ml, 41.00 ± 1.53 µg/ml, 61.50 ± 2.29 µg/ml and 71.75 ± 2.67 µg/ml respectively. Ethyl acetate fraction (ABE) exhibited superior antiglycation activity when compared to reference standard, aminoguanidine (IC50 value 51.25 ± 1.91 µg/ml). Figure 7 Antiglycation activities. Values are mean ± SEM (n = 3). ABL, lyophilized aqueous extract of A. bilimbi fruits; ABP, petroleum ether fraction of ABL; ABE, ethyl acetate fraction of ABL; ABB, butanol fraction of ABL; ABW, aqueous fraction of ABL; AG; amino guanidine.

3.4
According to Afolayan et al (2007), phenolic content of plant extracts could be responsible for their free radical scavenging activity and studies on fruits and vegetables also show a relationship between phenolic content and antioxidant activities (Omken et al., 2009). The antioxidant potential of polyphenols arises due to their high reactivity as hydrogen or electron donors, which can stabilize and delocalize the unpaired electron and chelate metal ions (Rice-Evans et al., 1997). In agreement with the above report, the ethyl acetate fraction (ABE) exhibited the highest phenolic content when compared with lyophilized aqueous extract, petroleum ether, butanol and aqueous fractions. These results suggested that the phenolic compounds significantly contributed to the antioxidant capacity of the Averrhoa bilimbi