TSA Or Complex Media
3. Would TSA be categorized as a defined or complex media?
TSA is categorized as a complex media because the digest of casein and soybean meal are presented in an undefined amount and the amount is varying for different batch of TSA which is what a complex media defined as (Sakazaki, 1978). Complex media is defined as media that contain components that are difficult to estimate within the media (Wessner et al., 2013).
4. Would you expect this media to support the growth of all bacteria?
It is not expected for all of bacteria to grow on TSA media but it would grow the vast majority of bacteria (Mahon et al., 2000). This is because some of the bacteria might require blood for its to grows and since TSA is made of soybean, it won’t be able to …show more content…
What type of media is KF streptococcal and what is the mode of action (i.e. what are the principle ingredients and how exactly does it work).
KF streptococcal is selective media because it contains chemicals that would suppress the growth of the undesirable bacteria (Lee et al., 1981). The media contain partially digested proteins which is known as peptone, that would dissolve in water and yeast extract, and also traces of Streptococci (Lee et al., 1981). There is also maltose and lactose present which is served as the power house which will provide the energy for the growth of the bacteria (VanMeter and Hubert, 2013). Furthermore, sodium azide within the media is used as the selective agents that would allow only the growth of the Gram-negative bacteria (VanMeter and Hubert, 2013).
8. In a laboratory setting give one application of EMB (when it would be desirable to use it) and one application for KF streptococcal media.
One application for EMB media would be selecting for Gram-negative bacteria against the Gram-positive bacteria and also comparing whether that bacteria have the ability to consume lactose or not (Sigma, 2014). And one application for KF streptococcal media would be whether if Streptococci presented in the media and it is a Gram-negative bacteria or not (Sigma,
TSA is categorized as a complex media because the digest of casein and soybean meal are presented in an undefined amount and the amount is varying for different batch of TSA which is what a complex media defined as (Sakazaki, 1978). Complex media is defined as media that contain components that are difficult to estimate within the media (Wessner et al., 2013).
4. Would you expect this media to support the growth of all bacteria?
It is not expected for all of bacteria to grow on TSA media but it would grow the vast majority of bacteria (Mahon et al., 2000). This is because some of the bacteria might require blood for its to grows and since TSA is made of soybean, it won’t be able to …show more content…
What type of media is KF streptococcal and what is the mode of action (i.e. what are the principle ingredients and how exactly does it work).
KF streptococcal is selective media because it contains chemicals that would suppress the growth of the undesirable bacteria (Lee et al., 1981). The media contain partially digested proteins which is known as peptone, that would dissolve in water and yeast extract, and also traces of Streptococci (Lee et al., 1981). There is also maltose and lactose present which is served as the power house which will provide the energy for the growth of the bacteria (VanMeter and Hubert, 2013). Furthermore, sodium azide within the media is used as the selective agents that would allow only the growth of the Gram-negative bacteria (VanMeter and Hubert, 2013).
8. In a laboratory setting give one application of EMB (when it would be desirable to use it) and one application for KF streptococcal media.
One application for EMB media would be selecting for Gram-negative bacteria against the Gram-positive bacteria and also comparing whether that bacteria have the ability to consume lactose or not (Sigma, 2014). And one application for KF streptococcal media would be whether if Streptococci presented in the media and it is a Gram-negative bacteria or not (Sigma,