Terminalia Tomentosa Lab Report

A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of ellagic acid, chlorogenic acid, gallic acid and quercetin in the leaf extract of Terminalia tomentosa and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of Butyl acetate: Formic Acid: Distilled Water 14:5:5 (v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.
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The plant is known to possess many pharmacological properties like antifungal ,antioxidantanti-hyperglycaemic , antidiarrhoeal, anti leucorrheal. From the literature survey, it is learnt that no substantial work has been carried out on the leaves of T.tomentosa in terms of physicochemical and preliminary phytochemical screening of T.tomentosa. However pertaining to our knowledge there is no any hyphenated HPTLC technique available anywhere else for simultaneous quantitation of ellagic acid, gallic acid, cholorogenic acid, quercetin and its formulation in methanolic extract. So the attempt has made to accept this challenge towards development and validation of ellagic acid, gallic acid, cholorogenic acid and quercetin simultaneously by such a hyphenated technology like HPTLC for the betterment of herbal quality
tomentosa was weighed in a round bottom flask. 100 ml of Methanol was added to the flask and the mixture was extracted by Soxhlate extraction after 12 hrs. The extract was then filtered through Whatman filter paper no. 41 (E. Merck, Mumbai, India and filtered through syringe filters of mesh size 0.45μ. The volume was made upto 100ml and used.

2.3.4 Formulation Sample
For analysis of the formulation sample 1 gm was accurately weighed into a round bottom flask. 30 mL of methanol was added to the flask and the mixture was refluxed on a boiling water bath for about 30 min. The extract was then filtered through Whatman filter paper no. 41 (E. Merck, Mumbai, India). The same procedure was performed twice and filtrate obtained was combined together and made up to 100 mL with methanol.

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