The Effect of an Enzyme on the Rate of a Reaction
Core Practical: Enzyme concentrations and enzyme activity.
Introduction
In this experiment I shall investigate how the enzyme concentration can affect the initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis for the experiment is that as the concentration of the enzyme is increased the rate of reaction will be increased, producing oxygen at a faster rate. The results in my opinion will show positive correlation. The amount of buffer solution (hydrogen peroxide) will be kept the same throughout the experiment in the interest of reliability and the amount of enzyme (potato) will be decreased as the experiment is carried out and a measurement will be taken as to how fast the enzyme is produced.
Variables
The independent variable for this investigation will be the amount of enzyme which will be reacting with the Hydrogen Peroxide solution. I will be using potato as the enzyme in the reaction and the amount of potato will begin at 5ml and decrease by 1ml increments until it reaches 1ml which will be the final measurement.
The dependent variable in this investigation will be the rate of the reaction between the enzyme and the buffer solution. According to my hypothesis the rate of reaction will decrease as the amount of enzyme is decreased and the reaction will take longer to produce a particular amount of oxygen as the amount of enzyme is decreased.
The controlled variables in my experiment are the amount of hydrogen peroxide which acts as the buffer solution, this is necessary in order to ensure accurate results. The boiling tube in which the reaction takes place will also be kept the same and will be cleaned out with water after each reaction. This is necessary to avoid
Introduction
In this experiment I shall investigate how the enzyme concentration can affect the initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis for the experiment is that as the concentration of the enzyme is increased the rate of reaction will be increased, producing oxygen at a faster rate. The results in my opinion will show positive correlation. The amount of buffer solution (hydrogen peroxide) will be kept the same throughout the experiment in the interest of reliability and the amount of enzyme (potato) will be decreased as the experiment is carried out and a measurement will be taken as to how fast the enzyme is produced.
Variables
The independent variable for this investigation will be the amount of enzyme which will be reacting with the Hydrogen Peroxide solution. I will be using potato as the enzyme in the reaction and the amount of potato will begin at 5ml and decrease by 1ml increments until it reaches 1ml which will be the final measurement.
The dependent variable in this investigation will be the rate of the reaction between the enzyme and the buffer solution. According to my hypothesis the rate of reaction will decrease as the amount of enzyme is decreased and the reaction will take longer to produce a particular amount of oxygen as the amount of enzyme is decreased.
The controlled variables in my experiment are the amount of hydrogen peroxide which acts as the buffer solution, this is necessary in order to ensure accurate results. The boiling tube in which the reaction takes place will also be kept the same and will be cleaned out with water after each reaction. This is necessary to avoid