When a substance is released into an area, the random movement of its molecules results in a multitude of collisions. These collisions, in turn, lead to a dispersion of the molecules. The overall movement of the molecules will be from an area of high concentration, where there will be more collisions, to areas of low concentration, where the number of collisions will be much less. This process of dispersion will continue until there is no net gain or loss of molecules in an area. The process by which this equilibrium occurs is called diffusion. Diffusion is vitally important to biology on many levels; individual cells, organelles, and even whole organisms rely on diffusion to carry out the processes essential to life. One especially important aspect of diffusion is osmosis, or the diffusion of water. This often occurs across a semi-permeable membrane…
The materials used in this experiment were a scale, 24" of dialysis tubing, 4 transfer pipettes, sugar, scissors, rubber bands, four coffee cups, a 250ml graduated cylinder, a ruler, a small sauce pan, and 3 clean containers.…
Fill 4 dialysis tubes 2/3 of the way with 1 M glucose solution. Fill 4 beakers with 100 mL of 1 M sucrose. Weigh all four bags before placing them into beakers. Record temperature of room temperature sucrose and place one bag into that beaker for ten minutes. After ten minutes, take the bag out, dry, and weigh. Heat up two beakers of sucrose solution to two different temperatures, record the temperatures, and place the bags in for ten minutes. After ten minutes, take the bags out, dry, and weigh. Cool down one beaker full of sucrose with ice, record the temperature, and place the last bag into the beaker. After ten minutes, take the bag out, dry, and weigh.…
1. The cell membrane regulates and controls what kind of molecules ______ move in & out of the cell.…
1. Cut four 6-inch pieces of dialysis tubing and soak in a coffee cup filled with tap water for 2 hours prior to your start time. While waiting, prepare the sugar solutions using the following protocol: you will need a saucepan, tap water and sugar near the stove for this portion of your experiment. Prepare the following three solutions:…
Seal the end if it is not already closed. Next, pick up a pipette and fill it with glucose. (Use this pipette only for glucose) then release the glucose into the dialysis tubing. Place four more pipettes full of glucose into the dialysis tubing. Now it is time to get another pipette (not the one used for glucose) and fill it with starch and release it into the dialysis tubing. Repeat this 4 (four) more times until there is a total of 5 pipettes of glucose and 5 pipettes of starch in the dialysis tubing. Mix the solution. Next, seal the dialysis tubing completely and make sure there is little to no air left in the dialysis tubing. Rise the bag with water. Next, fill a 400ml beaker with 300ml of water. Add 5 pipettes of Lugol´s into the 300ml of water. Mix the solution gently. Place the dialysis tubing bag into the 400ml beaker and leave for 30 minutes. After 30 minutes remove the bag and record your findings. Get 3 test tubes and label them “control” “bag” and “beaker” Next fill an unused pipette with water and pour it into the control test tube. Open the dialysis tubing and fill a pipette of the solution inside using an unused pipette. Repeat again and pour into the bag test tube. Place two pipettes of the beaker solution into the beaker tube. Add one pipettes of Benedict´s into each tube Heat all tubes for three minutes. Record…
Next, once the substances have been put into the tubes, with two finger, smooth the end of the tubing sso to release the excess air. Once that is done, proceed to clamp the other side encasing the solution. Make sure no leaks are present. Next, the team will proceed by placing each distinct dialysis tubing into the 6 beakers with distilled water before. Make sure the beakers and everything is labeled accordingly so no confusion is possible, as so:…
Diffusion and Osmosis are two concepts that go hand in hand with each other. Diffusion is simply described as the movement of a substance from a region of high concentration to a region of low concentration. In another words, the substance will move down its concentration gradient which is “the region along which the density of a chemical substance increases or decreases” (Campbell Biology pg. 132). If you understand the concept of diffusion then osmosis is a very simple process. It can be defined as the diffusion of water across a permeable membrane. Osmosis can be cellular or artificial, so even though we are creating artificial cells in this lab, it is still considered to be osmosis. During osmosis, a solvent is trying to get through a selectively permeable membrane to make the concentration of that solvent the same on both sides of the membrane. The rate of osmosis depends on the type of environment the cell is in. There are three different environments that a cell can find itself in, a hypertonic environment, a hypotonic environment, or an isotonic environment. The environment a cell is in will determine its tonicity which is “the ability of a surrounding solution to cause a cell to gain or lose…
These two Case Studies come from a National Center on Case Studies. I think that a case study approach is very useful in applying knowledge and this is what makes you learn it better. They may be a little daunting when you read them but I will help you go through them. Please ask for help so this topic becomes more enjoyable for you.…
We tied one end of the glucose tubing, and put 2 inches of glucose solution into it then we tied the other side into a knot to close it up. With the second dialysis tube we did the same thing but with the starch solution. We then weighed the glucose cell, then the starch cell, and recorded the mass into the chart. After that we placed each cell into a seperate cup, and filled it with water. We let the cells sit in the cups overnight. The next day we got our cup with the glucose cell, we took a glucose test strip and dipped it into the water in the cup to test for glucose. Then we took the cell out of the water, weighed it, and recorded it in the chart. We took the cup with the starch cell and tested it for starch by using lugols iodine. We put 4 drops of the iodine into the cup. We took the starch cell and weighed it, then recorded…
The dialysis tube was rinsed with tap water and the contents were emptied into a 100mL beaker.…
This makes sense because the farther the cell is from osmotic equilibrium, it will gain weight faster (McCutcheon 1926). This supported our prediction. The increase in osmotic rate because of an increase in solute concentration is because water moves from a solution of low solute concentration to a solution with high solute concentration. This means water rushes into a solute of higher concentration faster than a low concentration. This is why the artificial cell with 60% sucrose solution had the highest corrected cumulative change in weight and the fastest osmotic rate. The artificial cell containing water in 40% sucrose solution had a negative osmotic rate because water was leaving the cell and osmosis was occurring in the opposite direction. In this experiment, only one bag of water was placed in 40% sucrose solution. In other experiments, adding two more bags of water and placing them in 20% and 60% sucrose solutions could be tested to see if the rate of osmosis is similar to their 20% and 60% bag counterparts in water. Also, an experiment could be performed to show temperature is a factor in osmotic rates by having the same solute concentration in solutions and placing bags filled with water into them, each having a distinct…
Osmosis is the diffusion of a solvent across a selectively permeable membrane that occurs in response to differences in solute concentrations (Allen and Harper 2014).Osmosis can fall under the category of passive transport which does not require energy. With osmosis being a type of diffusion it is viewed as molecules moving from a high concentration to a low concentration. To further explain if there is a low water concentration, high amounts of solutes will be present. Water will most likely move to areas where the solute concentration is high, which demonstrates why there would be less water concentration.…
In this I will be investigating what effects the movement of water through osmosis. Osmosis is the diffusion of water. It is the process in which fluids pass through a partially-permeable membrane. It is the movement of water from high water concentration to low water concentration. Plant cells react to osmosis by hypertonic, isotonic and hypotonic.…
During the past few weeks in my Ag Biology class we have been learning about and reviewing both osmosis and diffusion. We did two different labs, one on osmosis; the other on diffusion.…