The Effect of Substrate Concentration on the Rate of Reaction
Method
I generally followed most of my plan for the experiments, but to improve accuracy and reliability, I made one change. In my plan, I said that I was going to use 20cm³ of starch for each experiment. However, in my actual experiment I used 40cm³ of starch. This is because I thought I might not have had enough solution if I only used 20cm³ of starch. If this was the case, then I would have to stop the experiment half way through, which would have prevented me from obtaining accurate results. So for this reason, my results would be more accurate and reliable. For everything else, I followed my original plan. To ensure the results were accurate, I made sure I accurately measured out the amylase and starch solutions for each experiment. This is because, if I had the wrong amounts, I would not get a valid conclusion, as I will not know whether my results are affected by the concentration of starch, or whether the results I got were due to the amounts of solution used. This would make both my results and conclusion invalid and unreliable. I also made sure that the temperature of the water bath was at the correct temperature and I kept on checking the temperature of both my amylase, and starch using a thermometer. I made sure both solutions were at the same temperature at all times throughout my experiments, so that temperature would not have an effect on my results. This would help me obtain more reliable and accurate results. Also, I made sure that I was drawing the solution up in the pipette beforehand, so that I could put it into the cuvette at exactly 30 seconds. This would make my results more accurate, as the solution would be put into the cuvette at precisely 30 seconds and so time would not have an effect on my results. I also used separate pipettes to measure out the starch, amylase and distilled water so that the solutions don’t mix together before the reaction should start. Also, I repeated each of my experiments two times to make sure I get accurate
I generally followed most of my plan for the experiments, but to improve accuracy and reliability, I made one change. In my plan, I said that I was going to use 20cm³ of starch for each experiment. However, in my actual experiment I used 40cm³ of starch. This is because I thought I might not have had enough solution if I only used 20cm³ of starch. If this was the case, then I would have to stop the experiment half way through, which would have prevented me from obtaining accurate results. So for this reason, my results would be more accurate and reliable. For everything else, I followed my original plan. To ensure the results were accurate, I made sure I accurately measured out the amylase and starch solutions for each experiment. This is because, if I had the wrong amounts, I would not get a valid conclusion, as I will not know whether my results are affected by the concentration of starch, or whether the results I got were due to the amounts of solution used. This would make both my results and conclusion invalid and unreliable. I also made sure that the temperature of the water bath was at the correct temperature and I kept on checking the temperature of both my amylase, and starch using a thermometer. I made sure both solutions were at the same temperature at all times throughout my experiments, so that temperature would not have an effect on my results. This would help me obtain more reliable and accurate results. Also, I made sure that I was drawing the solution up in the pipette beforehand, so that I could put it into the cuvette at exactly 30 seconds. This would make my results more accurate, as the solution would be put into the cuvette at precisely 30 seconds and so time would not have an effect on my results. I also used separate pipettes to measure out the starch, amylase and distilled water so that the solutions don’t mix together before the reaction should start. Also, I repeated each of my experiments two times to make sure I get accurate