INTRODUCTION
Calliphoridae- comonly known as a blowfly, are living organisms which usually are the first insects to colonise a body after death. The blowfly larvae are very useful in forensic entomology. The oldest larvae give an approximate time of death of body.
Family: Calliphoridae
Order: Diptera
Name: Calliphora vicina
Aim: To check the influence of temperature of environment on living functions and activity of Blow-fly larvae.
Hypothesis: If the temperature of environment is related to live activity of Blowfly larvae, then to high or low temperature will slow down or speed up the larvae activity.
DRAW
http://www.bumblebee.org/invertebrates/DipteraB.htm
APPARATUS:
1- Thermometer, 2- Stirrer, 3-500ml beaker, 4- 250ml beaker, 5- Retort stand, 6-Bunsen burner, 7- Asbestos mat, 8-forceps, 9- clamp and boss, 10- five blow-fly larvae
METHOD OF OBSERVATION
-Take the 250ml beaker and draw the line on the underside of the beaker.
-Fill 500ml beaker with tap water
-Put the smaller beaker into larger that the water will be in ¾ of large beaker and then clamp larger and smaller beakers using retort stand, boss and clamp.
-Put 5 maggots into smaller beaker using forceps.
-Use thermometer to check the environment temperature of maggots- put the thermometer into 250ml beaker and count how many times each larvae cross the line in each temperature. -Do the experiment for each temperature- 5 minutes.
- Use the ice or Bunsen burner to raise or low the temperature. Check the amount of line crossing for each larvae in temperature 10, 15, 20, 25, 30 C degrees. Do not overheat the maggots.
-While raise or low the temperature of larvae environment, start counting the lines crossed after 2 minutes to make sure that the temperature is stable.
-While the maggots start to climb on the wall, gently move them with thermometer bulb
- If