The Effects Of Various Environmental Co
The Effects of Various Environmental Conditions on the Rate of an Enzyme Catalyzed Reaction
Olivia Payne
Mr. Kuninsky
10/10/14
Variables:
1. Substrate Concentration
2. Substrate volume
3. Temperature
4. Enzyme Volume
5. Enzyme Concentration
6. pH
The independent variable my group addressed was temperature
The conditions for temperature were 5°C, 24°C, 35°C, and 45°C
We measured the independent variable using thermometers
The dependent variable was the rate of an enzyme catalyzed reaction
The dependent variable was measured by change in pressure
Purpose: General: The purpose of this lab was to determine the effect of environmental conditions on the rate of an enzyme catalyzed reaction. Specific: The purpose of this lab was to determine the effect of temperature on the rate of an enzyme catalyzed reaction.
Procedure: We followed the baseline procedure and made proper modifications for our independent variable of temperature. We prepared the tubing/stopcock apparatus before measuring out the catalase and hydrogen peroxide. After assembling the apparatus, we filled a syringe with 1 mL of catalase and placed it on ice. We then filled an Erlenmeyer flask with 10 mL of hydrogen peroxide and sat that in a 45°C water bath. We allowed the flask to sit in the water for three minutes before adding the catalase, so that the hydrogen peroxide could equilibrate. After three minutes we attached the stopper assembly to the flask and dispensed the catalase, making sure to close the stopcock after the catalase was dispensed in the flask. We then continued to collect data on a laptop. We used LoggerPro to collect and analyze the data.
Data:
H2O2 Concentration:
Condition
Initial Reaction Rate (kPa/sec)
0%
0.0009
1%
0.0777
2%
0.0888
3%
0.1395
H2O2 Volume:
Initial Reaction Rate (kPa/sec)
Condition
0mL
0.0288
1mL
0.1164
5mL
0.1420
10mL
0.1395
15mL
0.0498
20mL
0.0597
Temperature:
Condition
Initial Reaction Rate (kPa/sec)
5
0.1068
24
0.1395
35
0.1935
45
0.0698
Olivia Payne
Mr. Kuninsky
10/10/14
Variables:
1. Substrate Concentration
2. Substrate volume
3. Temperature
4. Enzyme Volume
5. Enzyme Concentration
6. pH
The independent variable my group addressed was temperature
The conditions for temperature were 5°C, 24°C, 35°C, and 45°C
We measured the independent variable using thermometers
The dependent variable was the rate of an enzyme catalyzed reaction
The dependent variable was measured by change in pressure
Purpose: General: The purpose of this lab was to determine the effect of environmental conditions on the rate of an enzyme catalyzed reaction. Specific: The purpose of this lab was to determine the effect of temperature on the rate of an enzyme catalyzed reaction.
Procedure: We followed the baseline procedure and made proper modifications for our independent variable of temperature. We prepared the tubing/stopcock apparatus before measuring out the catalase and hydrogen peroxide. After assembling the apparatus, we filled a syringe with 1 mL of catalase and placed it on ice. We then filled an Erlenmeyer flask with 10 mL of hydrogen peroxide and sat that in a 45°C water bath. We allowed the flask to sit in the water for three minutes before adding the catalase, so that the hydrogen peroxide could equilibrate. After three minutes we attached the stopper assembly to the flask and dispensed the catalase, making sure to close the stopcock after the catalase was dispensed in the flask. We then continued to collect data on a laptop. We used LoggerPro to collect and analyze the data.
Data:
H2O2 Concentration:
Condition
Initial Reaction Rate (kPa/sec)
0%
0.0009
1%
0.0777
2%
0.0888
3%
0.1395
H2O2 Volume:
Initial Reaction Rate (kPa/sec)
Condition
0mL
0.0288
1mL
0.1164
5mL
0.1420
10mL
0.1395
15mL
0.0498
20mL
0.0597
Temperature:
Condition
Initial Reaction Rate (kPa/sec)
5
0.1068
24
0.1395
35
0.1935
45
0.0698