When I apply four different seasonings (cinnamon, oregano, cumin, salt) to four cuts of the same brand of bologna, salt is going to have the strongest inhibitory effect and the bologna will not spoil.
Variables
Dependent Variable- The dependent variable of my experiment is the bologna meat.
Independent Variable- The independent variable of my experiment is the amount of seasoning that I will be applying to my dependent variable.
Constant Variable- The constant variable of my experiment is the agar used in my petri dishes and the brand of my dependent variable.
Materials
5 Petri Dishes (prefilled)
Clear desk tape
Journal (to record data)
1 slice of bologna
1/2 tsp of ground cinnamon
1/2 tsp of ground black pepper …show more content…
1/2 tsp of Kosher salt
1/2 tsp of ground cumin
Sterile gloves
Aluminum foil
1 plastic fork
4 plastic spoons
1 plastic knife
5 toothpicks
1 Sharpie marker
1 outdoor thermometer
Procedures
Wash and dry hands thoroughly
Roll out enough foil on which to place the slice of bologna (about 4 inches)
Put the sterile gloves on your hands to reduce the introduction of additional bacteria
Use a plastic fork to remove one slice of bologna from the package and place it on the aluminum foil
Use the plastic knife and fork to cut the bologna into 5 square inch pieces
Spread the 5 sections apart on the aluminum foil
Using a toothpick, place once piece of bologna into the center of a petri dish.
Place the lid over this sample. This will be used as the experiment control. Use clear desk tape to secure the lid to the petri dish.
For the 4 remaining bologna samples, sprinkle spice (ground black pepper, Kosher salt, ground cinnamon, or ground cumin) covering both sides of each sample individually. Care must be taken to use a different spoon with each spice to prevent cross-contamination of the sample.
Using a clean toothpick for each, place each spiced bologna sample into the center of each petri dish. Place the lid over the sample and secure with clear desk tape
Use a Sharpie to label each petri dish according to the spice and label the control.
Place the 5 samples into a secure container in a shaded area outdoors where they will not be disturbed. My samples will be placed on our private patio.
Record the date, the time, the outside temperature, and the condition of each of the 5 samples in the journal
Monitor the samples for 10 days recording the date, the time, the outside temperature and the condition of each of the 5 samples. If bacterial growth is present, record the appearance and size of bacteria by drawing a small sketch of the sample in the condition column of the
journal.