• Anti-Inflammatory: (1) VN significantly potentiated antiinflammatory activity of phenylbutazone and ibuprofen in albino rats.(2) Study suggests VN possess anti-imflammatory activity against acute and sub-acute inflammation probably due to prostaglandin inhibition and reduction of oxidative stress.
• Anti-Inflammatory / Analgesic: Study showed the fresh leaves of VN have anti-inflammatory and pain suppressing activities possibly mediated through PG synthesis inhibition, antihistmine, membrane stabilizing and antioxidant activities.
• Antibacterial / Essential Oil: Study showed the essential oils and extracts to have antibacterial activity. Essential oil and extracts showed promising results against B subtilis and E coli. Ethyl acetate and ethanol extracts showed prominent antibacterial activity against all tested strains.
• Antibacterial / Leaf, Flower and Fruits: Study of extracts of leaf, flower and fruit of VN was done to evaluate in vitro antibacterial activity against phytopathogens Pseudomonas solanacearum and Xanthomonas axonopodis. The ethyl acetate extract showed significant inhibition. Phytochemical screening yielded alkaloids, flavonoids, tannins, cardiac glycosides and terpenoids.
• Antifungal: (1) New antifungal flavonoid glycoside from Vitex negundo: Study found a new isolated flavone glycoside and a known compound to have significant antifungal activity against Tricophyton mentagrophytes and Cryptococcus neoformans. (2) Ethanol extract of fruit seeds showed significant activity against Fusarium solani and moderate response against Microsporum canis with no effect against C albicans.
• Larvicidal: Differential larvicidal efficacy of four species of Vitex against Culex quinquefasciatus larvae: The methanolic extracts of all Vitex species showed