The Scientific Method & Experimentation
Exercise 1
The Scientific Method & Experimentation
1. Title of Paper | The Antibacterial Activity of Crude Leaf Extract of Citrus sinensis (Sweet Orange) | 2. Author(s) | Uchechi N. Ekwenye and Oghenerobo V. Edeha | 3. Name of Journal | International Journal of Pharma and Bio Sciences | 4. Define the Problem | The antibacterial activity of crude leaf extracts of Citrus sinensis on 5 bacteria strains. | 5. State the hypothesis (es), if applicable | | 6. Statement of objectives | To evaluate the antibacterial activity of the crude leaf extract of Citrus sinensis against bacteria strains like Escherichia coli,Pseudomonas aeruginosa , Klebsiella pneumoniae, and Staphylococcus aureus.To be able to determine in which extract (aqueous or ethanol) the antibacterial capacity of Citrus sinensis is most effective. | 7. Variables used – independent and dependent | Independent Variable – Ethanol and Aqueous extracts Dependent Variable – Antibacterial activity | 8. Design of Experiment | | 9. Experimental Procedures | The in vitro antibacterial activity was performed by agar disc diffusion method. Muella Hinton agar was prepared and autoclaved at a specified temperature and pressure. The medium was then cooled and 20mL of the medium was poured into a sterile petri dish and allowed to solidify. A sterile wire loop was used to transfer a colony of test organisms into a test tube with 2mL peptide water. A swab stick was dipped into the test tube containing the organisms and it was used to seed the organism on the solidified Muella Hinton agar. The prepared disc was transferred on the the inoculated culture plates. The placed disc included 100mg/ml (DMSO) and gentamycin 2mg/ml.The plates were incubated for 24hours at 37oC, after which the zone of inhibition was measured and recorded. The test was carried out in duplicate. | 10. Analysis of data (results). What are the statistical tools used? | The ethanol extract of Citrus
The Scientific Method & Experimentation
1. Title of Paper | The Antibacterial Activity of Crude Leaf Extract of Citrus sinensis (Sweet Orange) | 2. Author(s) | Uchechi N. Ekwenye and Oghenerobo V. Edeha | 3. Name of Journal | International Journal of Pharma and Bio Sciences | 4. Define the Problem | The antibacterial activity of crude leaf extracts of Citrus sinensis on 5 bacteria strains. | 5. State the hypothesis (es), if applicable | | 6. Statement of objectives | To evaluate the antibacterial activity of the crude leaf extract of Citrus sinensis against bacteria strains like Escherichia coli,Pseudomonas aeruginosa , Klebsiella pneumoniae, and Staphylococcus aureus.To be able to determine in which extract (aqueous or ethanol) the antibacterial capacity of Citrus sinensis is most effective. | 7. Variables used – independent and dependent | Independent Variable – Ethanol and Aqueous extracts Dependent Variable – Antibacterial activity | 8. Design of Experiment | | 9. Experimental Procedures | The in vitro antibacterial activity was performed by agar disc diffusion method. Muella Hinton agar was prepared and autoclaved at a specified temperature and pressure. The medium was then cooled and 20mL of the medium was poured into a sterile petri dish and allowed to solidify. A sterile wire loop was used to transfer a colony of test organisms into a test tube with 2mL peptide water. A swab stick was dipped into the test tube containing the organisms and it was used to seed the organism on the solidified Muella Hinton agar. The prepared disc was transferred on the the inoculated culture plates. The placed disc included 100mg/ml (DMSO) and gentamycin 2mg/ml.The plates were incubated for 24hours at 37oC, after which the zone of inhibition was measured and recorded. The test was carried out in duplicate. | 10. Analysis of data (results). What are the statistical tools used? | The ethanol extract of Citrus