Preview

Thermal stability of Vitamin C in Fruit Juice

Better Essays
Open Document
Open Document
2806 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Thermal stability of Vitamin C in Fruit Juice
962/4 2013-2014

Title: Thermal stability of Vitamin C in Fruit Juice

Subtile: Effect of Temperature towards concentration of Vitamin C in Orange Juice

Members of the group:

Name
Identification number
Index Number
Chan Chyi Yong
950818-08-6787
SA2051/1001
Choong Jian Wen
950620-08-5279
SA2051/1005
Liew Sin Yung
950507-08-5373
SA2051/1011
Yong Yi Xiang
950510-02-5531
SA2051/1018

Teacher Advisor: Pn Kanahambikai

Date of Submit of this report:

Marks:

Abstract
Vitamin C or ascorbic acid is necessary for the human body, although only in small quantities, for acting as an essential supplement to a healthy diet as well as being a highly effective antioxidant, acting to lessen stress. People nowadays do not realize that preparing food under high temperature for a long period of time will decompose the Vitamin C contain in the food that is being prepared. This research aims to determine the concentration of Vitamin C after heating at (80oC) through a period of time by titrating the heated solution with DCPIP (2, 6-Dichloro-Indophenol) solution. In this experiment, Tropicana fruit juice beverage was used. 5ml of the fruit juice was measured using measuring cylinder then poured into boiling tube containing 25ml of oxalic acid. Then the solution is titrated with DCPIP solution, until the colour change from light orange to light pink. The volume of DCPIP needed to titrate is recorded. Then, another 5ml of Tropicana fruit juice and 25ml of oxalic acid prepared in a boiling tube was heated at 80oC for 5 minutes in a hot water bath. The solution is then titrated with DCPIP solution from orange to pink color. The volume of DCPIP needed for the titration process was recorded. The experiment is then repeated using time interval 20minutes, 35minutes, 50 minutes, and the volume of DCPIP needed to reach end-point is recorded. The concentration of Vitamin C is 0.48 mg cm- 3 at 0 minute, 0.48 mg cm-3 at 5 minute, 0.45

You May Also Find These Documents Helpful

  • Powerful Essays

    Lab 2 Autosaved

    • 1562 Words
    • 8 Pages

    In this experiment, we use titration method (volumetric analysis) to determine the centration of vitamin C in packaged fruit juice samples such as the mango, apple, passion fruit and orange juices in this experiment. Titration is a commonly used method of quantitative analysis which can determine the concentration of a known analyte (reactant). In this case the reactant is vitamin C. A titrant (reagent - Dichlorophenol-indophenol) of known concentration…

    • 1562 Words
    • 8 Pages
    Powerful Essays
  • Powerful Essays

    When you are satisfied that you have appropriate amounts you can carry out sufficient accurate titrations that will enable you to calculate the amount of vitamin C in your solution and to compare with the known…

    • 4606 Words
    • 19 Pages
    Powerful Essays
  • Better Essays

    Every fruit has a sweet taste only that some are sweeter than others. The sweetness of most fruits come from its sugar content and these sugars that the fruits contain are known as invert sugars. In this experiment, an orange was used. An orange which is an excellent source of vitamin C gets its sweetness from natural sugars which are sucrose, glucose and fructose (livestrong.com). In this experiment the concentration of glucose in an orange was measured. There are various methods which can be used to measure the glucose concentration but in this experiment a highly specific enzymatic method using the GOD-PAP assay (glucose oxidase peroxidase aminophenazone phenol) (schedule coventry,2013). This assay is based on 2 coupled enzyme reactions and a colorimetric end-point.…

    • 1441 Words
    • 5 Pages
    Better Essays
  • Better Essays

    Respectively, 1, 2, 4, 8, 16, and 24 drops of catechol were added to each of the tubes. To equal out the amount of solution in each of the test tubes, respectively 23, 22, 20, 16, 8 and 0 drops of pH 7 buffer were added. 30 drops of potato juice containing the catechol oxidase enzyme were then added to the solution, and then timed for 5 minutes at room temperature. The pH treatment only required 5 test tubes, with 3mL of matching buffer (pH4, pH6, pH7, pH8, and pH10) in the appropriate tube. Following this, 10 drops of both potato juice and catechol are added to the tubes, and then timed for 5 minutes at room temperature. For temperature, 6 test tubes were required, and placed in their appropriate temperature treatments all with 3mL of pH7 buffer in them. These tubes were place in either 3°C, 12°C, 20°C, 35°C, 50°C, and 70°C baths for 15 minutes to warm each tube up to the appropriate temperature. After the 15 minutes, 10 drops of both catechol and potato juice are added to each of the pH7 solutions.…

    • 1491 Words
    • 6 Pages
    Better Essays
  • Good Essays

    scientists and physicians have suggested doses up to 1 to 3 grams per day in order to help…

    • 785 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Lut1

    • 564 Words
    • 3 Pages

    This self-assessment form helps you determine your current level of readiness for this area of study. It is a starting point for you to begin preparation to demonstrate competency. While some of the topics listed on this form may seem new to you on first glance, keep in mind that the learning resources will help you gain an understanding of these topics. After filling out the self-assessment form, attend a “Getting Started” chat (details and times for the chats are located in the Learning Community) to learn more about developing competency in this area. If you are unable to attend a chat, would like a chat transcript, or would like further help interpreting this form, contact the course mentor for LUT1 via the “Contact a Mentor” icon in the course of study. In order to gain competency in this area, you should complete the activities listed in each week of the Course of Study (COS) and enroll in all available learning resources. The Course of Study (COS) can be found by going to your AAP and clicking on the Available Learning Resources link for this assessment. After completing the COS, you should return to the self-assessment form to determine progress made on development of the competencies.…

    • 564 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Daphnia are extremely small aquatic invertebrates. They are transparent, so you are able to see their internal organs; this means it is easy to monitor their heart rate by observing them through a microscope [1]. Physiological results from caffeine consumption include the stimulation of heart rate, blood pressure, metabolism, body temperature and alertness. [2] In this practical investigation the aim was to determine whether the hypothesis that caffeine increases the heart rate of Daphnia, was true or false or whether caffeine decreased the heart rate of Daphnia. To determine this you need to test this hypothesis practically; in order to do this you have to make up a range of caffeine solutions of different concentrations; this is the independent variable – the thing that you change during the investigation. You also need a control variable, which would be a solution which has no caffeine in at all, to compare the results from the caffeine solutions. In addition to this, you need a dependent variable; this is what you measure in the experiment and what is affected during the experiment, in the case of this investigation it would be the Daphnia’s heart rate which you would measure, and is affected by the independent variable – the concentration of caffeine solution.…

    • 1393 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Phosphate Lab Son

    • 2574 Words
    • 11 Pages

    The objective of this lab was to determine the concentration of phosphoric acid in cola through two different methods: a pH titration and a spectrophotometer method.…

    • 2574 Words
    • 11 Pages
    Good Essays
  • Better Essays

    Through the group results, seen in figure1, figure 2, and figure 3, the mean brought very similar absorption readings. The catechol oxidase with potato juice extract seemed to vary in production in pH data collected, though there was still gradual increase in production. Temperature solutions showed common production the 70- degrees F temperature and 3-degrees F temperature. As for the number of drops of catechol oxidase added to the solution proved that more of the enzyme present will lead to greater production of benzoquinone.…

    • 1271 Words
    • 6 Pages
    Better Essays
  • Good Essays

    Yarra Valley Essay

    • 1225 Words
    • 5 Pages

    In the Yarra Valley, the area around Healesville was initially occupied by the Yarra Yarra or Wurrundjeri Aboriginal group. By 1863 these people were settled at the Coranderrk Aboriginal Reservation on Badger Creek (figure 1), which soon became Victoria’s largest Aboriginal reserve until its closure in 1924. The Yarra Valley is one of the most important drainage basin and food production region in Australia. The area of Yarra Valley is approximately 5000 kilometers squared, and its border is located 25 kilometers of Melbourne’s CBD. It’s bordered by Whittleseas, Lilydale, Healesville etc. The Yarra catchment is depended on by most Melbourne’s 4 million people, for its water supply. The eastward expansion is encouraged by the Yarra Valley’s attractive landscape.…

    • 1225 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    The aim of this investigation is to discover the exact content of Vitamin C in a number of different common household products.…

    • 1747 Words
    • 7 Pages
    Powerful Essays
  • Satisfactory Essays

    Good food can make a person happy. Great food, friends and an inviting atmosphere can make for a perfect day. Yes, you can go to any number of restaurants and have a decent time, but if you want to treat your friend and self to a fantastic time, I highly recommend dining at Domingo’s in Mocksville, North Carolina.…

    • 155 Words
    • 1 Page
    Satisfactory Essays
  • Good Essays

    One of the greatest things about vitamins is that they can be taken in both pill and liquid form. Experts in nutrition consider liquid vitamins to be a remarkable breakthrough in the domain. This is one of the reasons which may explain the lately outburst of liquid supplement advertising.…

    • 1073 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    YOU WILL NOW DESIGN AN EXPERIMENT TO STUDY THE EFFECT OF A FACTOR AFFECTING VITAMIN C IN FRUIT JUICES…

    • 557 Words
    • 3 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Assumptions: Heating the test tubes for different periods of time provide environments where different amounts of heat are exerted onto the orange juice and the concentration of Vitamin C may be affected. It is suggested that the test tubes should hold amounts of fruit juice that are more than the amount of fruit juice needed to decolourize 3mL of DCPIP initially, as this allows investigation for whether more or less juice is needed, thus whether the concentration is decreased or…

    • 296 Words
    • 2 Pages
    Satisfactory Essays