Tissue Culture Technique for Callus Induction
Sarhad J. Agric. Vol. 23, No. 1, 2007
* Department of Plant Breeding and Genetics, NWFP Agricultural University, Peshawar – Pakistan.
** Agricultural Biotechnology Institute, National Agricultural Research Center, Islamabad – Pakistan.
TISSUE CULTURE TECHNIQUES FOR CALLUS INDUCTION IN RICE
Hidayat Ullah*, Iltaf Ullah*, Sultan Akbar Jadoon* and Hamid Rashid**
ABSTRACT
Seeds of two rice varieties Basmati-370 and Basmati-385 were evaluated for invitro callus induction at Agricultural
Biotechnology Institute in National Agricultural Research Center (NARC), Islamabad during 2001-02, using M.S and N6 media supplemented with 2, 4-D (2,4-Dichlorophenoxi acetic acid) and BAP (6-benzylaminopurine) at
@ 2.0, 2.5 and 0.1, 0.5 mgl-1 respectively. Objective of our study is to know the actual procedure for seed sterilization and to provide a well-adopted technique for callus induction in these varieties by using M.S and N6 media along with different concentrations of growth hormones. Seeds were used as explant source and to avoid chance of contamination sodium hypochlorite and ethanol at the rate of 50% and 70% were used respectively. Best response toward callus induction were observed for Bas-385 on both MS and N6 media however N6 media was proved to be best. Calli obtained from Bas-385 were friable and vigorous as compare to Bas-370. Bas-370 respond moderately on MS and N6 media at various combinations of 2,4-D and BAP. Bas-370 on MS media supplemented with 2,4-D and BAP @ 2.0 and 0.1 mgl-1 gave good performance towards callus induction. Over all results indicated that best callus were induced on MS and N6 when supplemented with 2,4-D and BAP @2.0 and 0.0 mgl-1 for variety Bas-385.
INTRODUCTION
In Pakistan rice is the second leading crop after wheat. In 2000-2001, the world annual production of rice was 598 million tones (FAO 2002). Asia is the largest producer of rice, with Bangladesh,
China, Philippines, Thailand and Vietnams as the leading rice
* Department of Plant Breeding and Genetics, NWFP Agricultural University, Peshawar – Pakistan.
** Agricultural Biotechnology Institute, National Agricultural Research Center, Islamabad – Pakistan.
TISSUE CULTURE TECHNIQUES FOR CALLUS INDUCTION IN RICE
Hidayat Ullah*, Iltaf Ullah*, Sultan Akbar Jadoon* and Hamid Rashid**
ABSTRACT
Seeds of two rice varieties Basmati-370 and Basmati-385 were evaluated for invitro callus induction at Agricultural
Biotechnology Institute in National Agricultural Research Center (NARC), Islamabad during 2001-02, using M.S and N6 media supplemented with 2, 4-D (2,4-Dichlorophenoxi acetic acid) and BAP (6-benzylaminopurine) at
@ 2.0, 2.5 and 0.1, 0.5 mgl-1 respectively. Objective of our study is to know the actual procedure for seed sterilization and to provide a well-adopted technique for callus induction in these varieties by using M.S and N6 media along with different concentrations of growth hormones. Seeds were used as explant source and to avoid chance of contamination sodium hypochlorite and ethanol at the rate of 50% and 70% were used respectively. Best response toward callus induction were observed for Bas-385 on both MS and N6 media however N6 media was proved to be best. Calli obtained from Bas-385 were friable and vigorous as compare to Bas-370. Bas-370 respond moderately on MS and N6 media at various combinations of 2,4-D and BAP. Bas-370 on MS media supplemented with 2,4-D and BAP @ 2.0 and 0.1 mgl-1 gave good performance towards callus induction. Over all results indicated that best callus were induced on MS and N6 when supplemented with 2,4-D and BAP @2.0 and 0.0 mgl-1 for variety Bas-385.
INTRODUCTION
In Pakistan rice is the second leading crop after wheat. In 2000-2001, the world annual production of rice was 598 million tones (FAO 2002). Asia is the largest producer of rice, with Bangladesh,
China, Philippines, Thailand and Vietnams as the leading rice