To compare the concentration of vitamin C in different brands of orange juice.
Practical Task:
To compare the concentration of vitamin C in different brands of orange juice.
Juice sample provided: 0.1% vitamin C solution, fresh orange juice, You C 1000 orange water.
Aim of investigation:
To compare and verify the amount of vitamin C in Mr. Juicy and You C 1000 orange juice.
Introduction:
Problem:
To compare the concentration of vitamin C
Hypothesis:
Concentration of vitamin C in Mr. Juicy is about 0.02 %
Concentration of vitamin C in You C 1000 orange water is about 0.1%
The concentration in You C 1000 orange water should be the highest, followed by fresh orange juice and Mr. Juicy orange juice.
Principle: DCPIP food test is conducted. Vitamin C can reduce the blue dye DCPIP (Dichlorophenol-indophenol) and turn its colour from blue to colourless. This process is known as decolourization. Thus, we can estimate the amount of Vitamin C in the brands of orange juice by comparing:
1.) The volume of vitamin C solution (of 0.1% concentration) needed to decolourize a certain volume of DCPIP solution.
2.) The volume of a certain brand of orange juice needed to decolourize the same volume of a known concentration of DCPIP solution.
3.) Variable table:
Independent Variable Dependent Variable Controlled Variable
Different brands of orange juice Concentration of 0.1 % vitamin C solution Same amount of DCPIP solution 4.) Assumption: Assume that the temperature of the orange juice are the same so there won’t be experimental errors.
Apparatus and materials required:
(In groups of two)
Test-tube x10
Test-tube rack x2
Pipette x1
Pipette filler x1
Dropper x4
Measuring cylinder x2
0.1% Vitamin C (ascorbic acid) solution (1000mg/1L)
Fresh orange juice
Mr. Juicy orange juice
You C 1000 orange water
Procedure:
1.) Use a pipette to put 1mL of DCPIP solution (blue) in a test-tube.
2.) Use a dropper to add 0.1% vitamin C solution, drop by drop, to the DCPIP solution. Stop once noted any colour changes. Record the
To compare the concentration of vitamin C in different brands of orange juice.
Juice sample provided: 0.1% vitamin C solution, fresh orange juice, You C 1000 orange water.
Aim of investigation:
To compare and verify the amount of vitamin C in Mr. Juicy and You C 1000 orange juice.
Introduction:
Problem:
To compare the concentration of vitamin C
Hypothesis:
Concentration of vitamin C in Mr. Juicy is about 0.02 %
Concentration of vitamin C in You C 1000 orange water is about 0.1%
The concentration in You C 1000 orange water should be the highest, followed by fresh orange juice and Mr. Juicy orange juice.
Principle: DCPIP food test is conducted. Vitamin C can reduce the blue dye DCPIP (Dichlorophenol-indophenol) and turn its colour from blue to colourless. This process is known as decolourization. Thus, we can estimate the amount of Vitamin C in the brands of orange juice by comparing:
1.) The volume of vitamin C solution (of 0.1% concentration) needed to decolourize a certain volume of DCPIP solution.
2.) The volume of a certain brand of orange juice needed to decolourize the same volume of a known concentration of DCPIP solution.
3.) Variable table:
Independent Variable Dependent Variable Controlled Variable
Different brands of orange juice Concentration of 0.1 % vitamin C solution Same amount of DCPIP solution 4.) Assumption: Assume that the temperature of the orange juice are the same so there won’t be experimental errors.
Apparatus and materials required:
(In groups of two)
Test-tube x10
Test-tube rack x2
Pipette x1
Pipette filler x1
Dropper x4
Measuring cylinder x2
0.1% Vitamin C (ascorbic acid) solution (1000mg/1L)
Fresh orange juice
Mr. Juicy orange juice
You C 1000 orange water
Procedure:
1.) Use a pipette to put 1mL of DCPIP solution (blue) in a test-tube.
2.) Use a dropper to add 0.1% vitamin C solution, drop by drop, to the DCPIP solution. Stop once noted any colour changes. Record the