Trimer 12a Lab Report

Trimer 9a and Dimer 12a Effect on Fibrin Production for Thrombin Activity Allosteric inhibitors are used to block an active site of an enzyme so the substrate is unable to bind.1 In this study, trimer 9a and dimer 12a are competitive inhibitors that inhibit fibrin production at different rates per minute depending on concentration of the inhibitor present (Figures 1A, B). Since the inhibitor concentration alters Michaelis-Menten kinetics, it is determined trimer 9a and dimer 12a are allosteric inhibitors.2 Trimer 9a has an inhibiting effect on fibrin production when concentration is increased. When a concentration of 30 µM of trimer 9a was used at a