Two Ways That Are Naturally Competent And Artificial Transformation

Transformation can occur in two ways that are naturally competent and artificial competent. Natural competent is the ability of the cell to take up DNA from the environment. Whereas, the artificial transformation is done to any types of cell that cannot take up DNA by themselves. The example of artificial transformation is by heat shock method. Heat shock method is a method that gives an electrical charge that can open pores from the cell membrane of the E. coli. When the pores are open, the plasmid DNA can be inserted into the bacterial cells. In this experiment, three Eppendorf tubes were used. Tube A contains pUC19. Tube B contains ligation mixture (LM) that contains SOD and pUC19 and tube C contain competent cells. This procedure was done