Unknown Enteric Bacteria Report
Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube
Brooke Addario
2/16/2015
BIO 488C - Section J
TA – Jacob Karsten
Introduction:
Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative, rod shaped, facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however, they are also a normal part of human bacterial flora and are therefore found in both healthy and infected hosts. This is why fecal matter found in contaminated water and food supplies as well as public recreational areas such as beaches can cause serious intestinal tract infections and urinary tract infections, among other diseases (Todar 2012). Escherichia coli is a major …show more content…
The first day, materials included 1 Enteropluritube and a sample culture of the unknown Enterobacteriaceae. In order to inoculate the tube, both caps were first removed from either end of the tube. One end of the tube will reveal a short exposed metal tip of a wire that runs through the Enteropluritube. This metal inoculating tip was used to gather one isolated colony of unknown enteric bacteria by touching the end to a colony. The opposite end (the side closest to the glucose test) of the tube was then twisted and pulled out, gradually twisting the wire through the tube, taking care not to pull it out entirely. The wire was then reinserted the same way it was pulled out until the tip came back out the other end. A notch became visible at this end allowing the tip to be snapped off. The snapped of tip was then used to puncture holes in the plastic film on the tube’s side covering the last eight tests (adonitol, lactose, arabinose, sorbitol, Voges-Proskauer, dulcitol/PA, urea, and citrate). Both caps were then replaced, and the tube was incubated for 24 hours at 37 °C (Hébert and Leid and Shand
Brooke Addario
2/16/2015
BIO 488C - Section J
TA – Jacob Karsten
Introduction:
Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative, rod shaped, facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however, they are also a normal part of human bacterial flora and are therefore found in both healthy and infected hosts. This is why fecal matter found in contaminated water and food supplies as well as public recreational areas such as beaches can cause serious intestinal tract infections and urinary tract infections, among other diseases (Todar 2012). Escherichia coli is a major …show more content…
The first day, materials included 1 Enteropluritube and a sample culture of the unknown Enterobacteriaceae. In order to inoculate the tube, both caps were first removed from either end of the tube. One end of the tube will reveal a short exposed metal tip of a wire that runs through the Enteropluritube. This metal inoculating tip was used to gather one isolated colony of unknown enteric bacteria by touching the end to a colony. The opposite end (the side closest to the glucose test) of the tube was then twisted and pulled out, gradually twisting the wire through the tube, taking care not to pull it out entirely. The wire was then reinserted the same way it was pulled out until the tip came back out the other end. A notch became visible at this end allowing the tip to be snapped off. The snapped of tip was then used to puncture holes in the plastic film on the tube’s side covering the last eight tests (adonitol, lactose, arabinose, sorbitol, Voges-Proskauer, dulcitol/PA, urea, and citrate). Both caps were then replaced, and the tube was incubated for 24 hours at 37 °C (Hébert and Leid and Shand