Lab Report 1
Water Flow Across Semi-Permeable Membranes
WATER FLOW ACROSS CELL MEMBRANES pg.2
WATER FLOW ACROSS CELL MEMBRANES
The movement of water across semi-permeable membranes as it relates to the laws of thermodynamics and energy flow with the diffusion of molecules down a concentration gradient of various solute concentrations, observing microscopically viewed outcomes, and spectrophotometrically measured results.
ABSTRACT
This experiment will identify the movement and exchange of water through semipermeable membranes and the recognizable amount of cellular destruction possible as a result in this change of environment. Tested were different samples of mammalian red blood cells (RBC) which were microscopically analyzed and measured for absorbency rates with a spectrophotometer when introduced to the various solute concentrations of 0.6% salt (Na), 0.897% Na, 5.0% Na, and distilled water with a 0.0% Na solute concentration. My hypothesis is that cellular form and contents will either change or maintain form and consistency when introduced to these various different environments as osmosis occurs until a dynamic equilibrium has been reached between the membrane and its environment. It was found that when RBC’s are introduced to various solute concentrates their form and consistency did changed with highest amount of hemolysis evident in the distilled water 0% Na concentration with an absorbency rate of 2.091% and the lowest amount of hemolysis occurring
WATER FLOW ACROSS CELL MEMBRANES pg.3 in the 0.897% Na or isotonic solution with an absorbency rate of 1.657%. Therefore it can be said that water will always move