This experiment was designed to answer the question does temperature affect the amount
of osmosis? The hypothesis predicted was that the higher the temperature the more osmosis
would occur, but too high the osmosis would halt due to enzyme and substrate overheating and
losing shape. After research and class time it was concluded that osmosis is a passive transport
and would not require energy or enzymes due to it going from high to low concentrations with
the gradient. The procedure was that four beakers would be labeled number one through four and
each would have a different temperature. One for a low temperature, one for room temperature,
one for medium and finally one to test a high near boiling temperature. The water was measured
to 200 mL and each cell had a cell placed inside it made from dialysis tubing. The cells had 10
mL of sucrose within tied together or clamped tight to preserve the solution. The temperatures
and initial cell mass recorded, the experiment began and was timed for 30 minutes. After that
time, the cells were removed and final masses recorded, and the percent change was calculated.
The conclusion developed from the data and knowledge was that the higher the temperature of
the water, the more osmosis occurred.
Introduction
Osmosis is the diffusion of water through a selectively permeable membrane from an area
which is more concentrated to less concentrated. The objective of this lab was to determine if
certain factors affect the amount of osmosis through a cell membrane, which in other words is
how much water is passing through the membrane with the process of Osmosis. Temperature
was the factor chosen to test its effect upon osmosis, questioning would the temperature of
water affect the amount of Osmosis occurring? The hypothesis formed that the higher the water
temperature is, the more osmosis will occur. But, too high of a temperature the