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2. A single tube of cerebrospinal fluid is received in the laboratory and the following tests requested: total protein, albumin, IgG quantitation, microbial culture, Gram stain, leukocyte count and differential cell count. The specimen should be sent to the various laboratories in which order?

a. Chemistry lab, hematology lab, microbiology lab
b. Hematology lab, chemistry lab, microbiology lab
c. Microbiology lab, hematology lab, chemistry lab
d. Hematology lab, microbiology lab, chemistry lab

3. You need to prepare a 1 to 4 (1:4 or ¼) dilution of a serum specimen using saline before analysis. Which of the following pipetting steps would result in this dilution?

a. Pipet 0.5 mL serum, then add 1.0 mL saline
b. Pipet 1.0 mL serum, then add 2.0 mL saline
c. Pipet 1.5 mL serum, then add 4.5 mL saline
d. Pipet 2.0 mL serum, then add 5.0 mL saline

4. The major components of a spectrophotometer are represented in the following diagram. Which component determines the factor “b” in Beer’s law, A=abc?

A B C D

a. Component A
b. Component B
c. Component C
d. Component D

5. At pH of 7.4, which of the enzymes listed catalyzes the following reaction?

Pyruvate + NADH lactate + NAD

a. Lactate oxidase
b. Lactate dehydrogenase
c. Pyruvate kinase
d. Pyruvate decarboxylase

6. pH 8.6 is used for serum protein electrophoresis so that

a. All serum proteins will have a net negative charge
b. All serum proteins will have a net positive charge
c. Electroendosmosis is avoided
d. Heat production is minimized

7. Osmolality measurements determine the

a. Activity of ions per kilogram of solvent
b. Grams of dissolved solutes per kilogram of solvent
c. Moles of dissolved solutes per kilogram of solvent
d. Equivalents of dissolved solutes per kilogram of solvent

8. Which of the following formulas can be used to calculate serum