Week 2 Experiment Answer Sheet
WEEK 2 EXPERIMENT ANSWER SHEET
Please submit to the Week 2 Experiment dropbox no later than Sunday midnight.
SUMMARY OF ACTIVITIES FOR WEEK 2 EXPERIMENT ASSIGNMENT
Experiment 2 Exercise 1a – Effect of substrate concentration on enzyme function
Experiment 2 Exercise 1b – Effect of pH on enzyme function
Experiment 2 Exercise 2 – Cellular Respiration and Photosynthesis
Experiment 2 Exercise 1A: Effect of substrate concentration on enzyme function
Review the Week 2 Experiment Introductions, our online lecture on Energetics and pp 80 - 82 in your book. For this exercise, we are going to look at the effect of substrate concentration on enzyme function, while holding enzyme concentration, pH and temperature constant. Open the following website to get started:
Glencoe- McGraw Hill. No date. Enzyme-controlled Reactions http://glencoe.mcgraw-hill.com/sites/dl/free/0078759864/383930/BL_11.html Procedure
A. Click on the TV/VCR and listen to the short overview about enzyme action. Close the window when done.
B. Then click on the Information button and review the information there. Close the window when done.
C. In our first experiment, we will determine the effect of different amounts of substrate on enzyme function. First, we need to set up the experiment:
a. Click on the 0.5 g substrate weigh paper and drag it to Tube #1; note the color change. When our enzyme breaks down the substrate, the solution turns blue. The intensity of the color indicates the amount of product formed.
b. Now drag the 1.0 g substrate weigh paper to Tube #2 and continue as such with the rest of the substrate samples as indicated in the table below.
c. Click on the computer monitor to view your results and transcribe them to Table 1 below.
Table 1. Substrate amount and product formation results (1 pts).
Test Tube Number
Amount of Substrate (g)
# of Molecules of Product Formed per Minute (X 106)
1
0.5
72
2
1.0
145
3
2.0
300
4
4.0
350
5
8.0
350
D. Generate a scatter plot of Amount of Substrate
Please submit to the Week 2 Experiment dropbox no later than Sunday midnight.
SUMMARY OF ACTIVITIES FOR WEEK 2 EXPERIMENT ASSIGNMENT
Experiment 2 Exercise 1a – Effect of substrate concentration on enzyme function
Experiment 2 Exercise 1b – Effect of pH on enzyme function
Experiment 2 Exercise 2 – Cellular Respiration and Photosynthesis
Experiment 2 Exercise 1A: Effect of substrate concentration on enzyme function
Review the Week 2 Experiment Introductions, our online lecture on Energetics and pp 80 - 82 in your book. For this exercise, we are going to look at the effect of substrate concentration on enzyme function, while holding enzyme concentration, pH and temperature constant. Open the following website to get started:
Glencoe- McGraw Hill. No date. Enzyme-controlled Reactions http://glencoe.mcgraw-hill.com/sites/dl/free/0078759864/383930/BL_11.html Procedure
A. Click on the TV/VCR and listen to the short overview about enzyme action. Close the window when done.
B. Then click on the Information button and review the information there. Close the window when done.
C. In our first experiment, we will determine the effect of different amounts of substrate on enzyme function. First, we need to set up the experiment:
a. Click on the 0.5 g substrate weigh paper and drag it to Tube #1; note the color change. When our enzyme breaks down the substrate, the solution turns blue. The intensity of the color indicates the amount of product formed.
b. Now drag the 1.0 g substrate weigh paper to Tube #2 and continue as such with the rest of the substrate samples as indicated in the table below.
c. Click on the computer monitor to view your results and transcribe them to Table 1 below.
Table 1. Substrate amount and product formation results (1 pts).
Test Tube Number
Amount of Substrate (g)
# of Molecules of Product Formed per Minute (X 106)
1
0.5
72
2
1.0
145
3
2.0
300
4
4.0
350
5
8.0
350
D. Generate a scatter plot of Amount of Substrate