What Happens When The Frog Muscle Was Heated For Too Long

The results that were obtained are nothing similar to the expected results, the whole experiment was a total fail. The frog muscle was heated for too long, so it stiffened before any recording of the results by the machine occurred, and there’s also a high probably that the muscle that we assume to have been exposed to heat for too long, probably died before the experiment commenced; the reason behind this is that one of the experimenters held the muscle in her bare hand(that was probably salty because of sweat, and because of this it is safe to assume that it was a hypertonic environment relative to the muscle cells) while the equipment was being set up. To add further to the failing of the experiment, very small amounts of Ringer were used during the period when we waited for the equipment to be set up. When the muscle was attached to the kymograph, a flat line was observed, until the tension generated by the added wait. The expected that the solidification of the muscle proteins was going to occur above a temperature of 38 °C. It started off with myosin, followed tropomyosin, and then finally troponin, at 45 °C, 47 °C, and 56 °C respectively.
The consequence of exposing the muscle to a hypertonic environment, and not using enough Ringer before the experiment began, went beyond just shrinking the muscle, it also abolished any type of twitching- because of the dislodging of the myosin from its position in the muscle’s contractile unit (Mirsky, 1937). This could explain why we couldn’t obtain any readings from the muscle, so even though it might not have been dead at some point during the experiment, obtaining the results was going to be impossible because we had damaged the muscle’s contractile abilities sooner than we should