What Is 3.13 Test For Saponin Glycosids?

3.13 Test for Saponin glycosides 2g of dried plant powder add 5ml of distill water shake vigorously. Persistent of foam indicate the presence of saponin glycosides. 3.14 Test for Steroids (Salkowski's test) 5ml of test solution add with 2ml of Chloroform and then Conc. H2SO4 sides of the test tubes. The appearance of color at the interface indicates the presence of steroids.

3.15 Experimental Procedure for preliminary Phytochemical studies (Brindha et al., 1981)

S. No
Name of the Test
Experimental Procedure
Observation

1
Test for Carbohydrates: Molisch’s test 2-3ml of extract, add two drops of alpha naphthol solution in alcohol shake and add conc. H2SO4 from sides of test tube.
Violet coloration occurs.

2
Test for
The UV – VIS spectrum analysis of ethanol leaf extract of Diplocyclos palmatus. was carried out St. Joseph’s College, Tiruchirappalli – 2. Using a perkin Elmer spectrophotometer system. Were used at wavelength from 400 to 800 nm due to sharpness of the peaks and proper baseline. The profile showed the peaks at 422 and 644 nm with the absorption of respectively solvent. Sahaya Sathis, S et al., (2012).
3.17 Fourier Transform Infrared Spectroscopy (FT- IR)
The FT – IR spectrum was used to identify the functional group of the active components based on the peak values in the region of infrared radiation. FT - IR analysis was carried out St. Joseph’s College, Tiruchirappalli – 2. Using a Perkin Elmer spectrophotometer system (Model: spectrum RX I) at the range of 4000 cm-1 to 400 cm-1 which was used to detect the characteristic peaks and their functional groups. The peaks values of the FT - IR were recorded. The results of peak values and functional groups. Were represented in Table- 7. Neha Sahu and Jyoti Saxena
Mass spectra were taken at 7- eV; a scan interval of 0.5 s and fragments from 45 to 450 Da. The solvent delay was 0 to 2 min and the total GC MS running time was 36 min. The samples were injected in split mode as 10:1 Mass spectral scan range was set at 45 to 450 (m/z). The mass detector used in thesis analysis was clarus 580 perkin Elmer and the software adopted to handle mass spectra and chromatograms was a tuber Mass ver 5.2 (Imade, H H et al., 2015b; Mohammed et al.,