Why Did We Mix The Plant Suspension With Meat Tenderizer
1. (3 pts) Describe the chemical structure of the plant cell wall and plant cell membrane. Make sure to include whether each structure is hydrophobic or hydrophilic.
The plant cell wall contains cellulose and the membrane contains phospholipids. Cellulose is hydrophilic, meaning that it likes and is attracted to water. The phospholipid bilayer that makes up the cell membrane is both hydrophilic and hydrophobic. It contains hydrophilic heads that face the outside of the bilayer, and hydrophobic tails that face the inside of the membrane. The heads are therefore polar and the tails are nonpolar.
2. (2 pts) Why would a cell contain enzymes (like endonucleases) that damage or destroy DNA?
A cell probably contains endonucleases as a form of …show more content…
(2 pts) Which aspect of this experiment enables us to lyse the cell membrane?
Both of these components can be broken down using detergent, which releases the components inside of the outer covering. The detergent essentially destroys the cell wall and the cell membrane. All contents inside of the cell are released which includes organelles, proteins, and DNA.
4. (2 pt) Why did we mix the plant suspension with meat tenderizer?
The meat tenderizer acted as a protease in the experiment. The job of a protease is to disable the action of the nucleases. The nucleases attack nucleotides, and therefore damage the DNA. When a cell lyses and all of its contents are released, there are DNA damaging proteins that will be released that are typically separated from DNA. These proteins are the nucleases. We used the meat tenderizer as a way to stop the nucleases from damaging the DNA before we were able to separate it out of the solution.
5. (5 pts) Perform the human DNA extraction linked on the lab webpage.
What did this experiment use instead of meat tenderizer to remove proteins?
a. The experiment used Proteinase K in order to remove proteins.
What was the purpose of the salt
The plant cell wall contains cellulose and the membrane contains phospholipids. Cellulose is hydrophilic, meaning that it likes and is attracted to water. The phospholipid bilayer that makes up the cell membrane is both hydrophilic and hydrophobic. It contains hydrophilic heads that face the outside of the bilayer, and hydrophobic tails that face the inside of the membrane. The heads are therefore polar and the tails are nonpolar.
2. (2 pts) Why would a cell contain enzymes (like endonucleases) that damage or destroy DNA?
A cell probably contains endonucleases as a form of …show more content…
(2 pts) Which aspect of this experiment enables us to lyse the cell membrane?
Both of these components can be broken down using detergent, which releases the components inside of the outer covering. The detergent essentially destroys the cell wall and the cell membrane. All contents inside of the cell are released which includes organelles, proteins, and DNA.
4. (2 pt) Why did we mix the plant suspension with meat tenderizer?
The meat tenderizer acted as a protease in the experiment. The job of a protease is to disable the action of the nucleases. The nucleases attack nucleotides, and therefore damage the DNA. When a cell lyses and all of its contents are released, there are DNA damaging proteins that will be released that are typically separated from DNA. These proteins are the nucleases. We used the meat tenderizer as a way to stop the nucleases from damaging the DNA before we were able to separate it out of the solution.
5. (5 pts) Perform the human DNA extraction linked on the lab webpage.
What did this experiment use instead of meat tenderizer to remove proteins?
a. The experiment used Proteinase K in order to remove proteins.
What was the purpose of the salt